期刊
BIOSCIENCE REPORTS
卷 41, 期 8, 页码 -出版社
PORTLAND PRESS LTD
DOI: 10.1042/BSR20211060
关键词
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资金
- Japan Society for the Promotion of Science [JSPS KAKENHI] [18K07461]
- Grants-in-Aid for Scientific Research [18K07461] Funding Source: KAKEN
The study investigated the redox status of Cys-thiol in serum apolipoprotein E and found a correlation between the number of Cys residues and the ratios of different apoE types. The results contribute to a better understanding of the redox biology of age-related diseases.
Background: The redox modulation of Cys-thiol participates in various pathophysiological processes. We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named redox-IDX-apoE, which is necessary to understand the redox biology of age-related diseases. Methods: The fractions of the reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE in serum, obtained from the patients with no apparent disease (controls, n=192) and with atherosclerosis and type 2 diabetes (patients, n=16), were measured by a band-shift assay using a maleimide compound. Redox-IDX-apoE candidates were determined by calculating the values of these fractions and the total apoE concentration. Results: Cys number of apoE significantly increased for the ratio of roxi-apoE to total-apoE (roxi/total) (E2/E3>E3/E3>E3/E4) but decreased for the ratios of red-apoE to roxi-apoE (red/roxi) and [red-apoE + oxi-apoE] to roxi-apoE ([red + oxi]/roxi) (E2/E3
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