期刊
BIORESOURCE TECHNOLOGY
卷 332, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125150
关键词
Astaxanthin; Carotenogenesis; H. pluvialis; Transcriptome sequencing; Metabolic pathways
资金
- Intramural Action of the Spanish National Research Council [201420E035, BIO2017-84066-R]
- Spanish Ministry of Science and Innovation
Research has advanced in understanding the synthesis and accumulation of astaxanthin in Haematococcus, revealing transcriptional activation of key enzymes involved in providing precursors for carotenoids and fatty acids, as well as identification of potential transcriptional regulators to control astaxanthin accumulation for optimizing commercial production.
Astaxanthin is a valuable and highly demanded ketocarotenoid pigment, for which the chlorophycean microalga Haematococcus pluvialis is an outstanding natural source. Although information on astaxanthin accumulation in H. pluvialis has substantially advanced in recent years, its underlying molecular bases remain elusive. An integrative metabolic and transcriptomic analysis has been performed for vegetative Haematococcus cells, grown both under N sufficiency (green palmelloid cells) and under moderate N limitation, allowing concurrent active cell growth and astaxanthin synthesis (reddish palmelloid cells). Transcriptional activation was noticeable in reddish cells of key enzymes participating in glycolysis, pentose phosphate cycle and pyruvate metabolism, determining the adequate provision of glyceraldehyde 3 phosphate and pyruvate, precursors of carotenoids and fatty acids. Moreover, for the first time, transcriptional regulators potentially involved in controlling astaxanthin accumulation have been identified, a knowledge enabling optimization of commercial astaxanthin production by Haematococcus through systems metabolic engineering.
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