4.8 Article

Methane-based biosynthesis of 4-hydroxybutyrate and P (3-hydroxybutyrate-co-4-hydroxybutyrate) using engineered Methylosinus trichosporium OB3b

期刊

BIORESOURCE TECHNOLOGY
卷 335, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125263

关键词

Methane; Methanotrophs; 4-Hydroxybutyric acid; P(3-hydroxybutyrate-co-4-hydroxybutyrate); Methylosinus trichosporium OB3b

资金

  1. C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2015M3D3A1A01064882]

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This study successfully synthesized 4-HB in Type II methanotrophs Methylosinus trichosporium OB3b and increased the yield to 10.5 mg/L. By combining different metabolic pathways, the engineered strains were able to synthesize P(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer with 3.08 mol% 4-HB from methane as the sole carbon source.
4-Hydroxybutyric acid (4-HB) is a key platform chemical that serves as a precursor in a wide variety of industrial applications including 1,4-butanediol and bioplastics production. In this study, we reconstructed 4-HB biosynthetic pathway including CoA-dependent succinate semialdehyde dehydrogenase and NADPH-dependent succinate semialdehyde reductase in Type II methanotrophs, Methylosinus trichosporium OB3b, to synthesize 4-HB. These engineered strains were able to synthesize 4-HB from methane via tricarboxylic acid cycle. 4-HB synthesis was further improved to 10.5 mg/L by overexpressing phosphoenolpyruvate carboxylase, isocitrate dehydrogenase and 2-oxoglutarate dehydrogenase genes in M. trichosporium OB3b. We combined the native poly(3hydroxybutyrate) metabolic pathway and reconstructed 4-HB biosynthetic pathway to synthesize P(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer from structurally unrelated substrate methane as a single carbon source. These engineered strains could synthesize P(3HB-co-4HB) copolymer with 3.08 mol% 4-HB from methane. This study provides several engineering strategies to synthesize polyhydroxyalkanoates and their monomers from methane.

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