Evaluating the effect of fluoxetine on mesophilic anaerobic dark biohydrogen fermentation of excess sludge

Recently, the influence behavior of new pollutants in the environment has been widely concerned. However, the effect of antidepressants widely detected in excess sludge (ES) on biohydrogen production from anaerobic dark fermentation has never been explored. To fill this gap, fluoxetine (FLX), a typical antidepressant, was selected to evaluate its effect on ES mesophilic anaerobic dark biohydrogen fermentation. The results showed that FLX reduced biohydrogen production even at low content (0.1 mg/Kg). The biohydrogen yield was only 12.8 mL/g in the 1.8 mg/Kg (based on total suspended solids) FLX group, decreased by about 34.7%, compared with the control group (without FLX). Further mechanism investigation implied that high levels (more than 0.6 mg/Kg) of FLX reduced every step associated with the biohydrogen production. FLX reduced the concentration of ammonia nitrogen and phosphate in fermentation broth. FLX also had a significant negative effect on enzyme activity in ES dark fermentation.

Automated summary

Fluoxetine inhibits biohydrogen production in anaerobic dark fermentation, even at low concentrations. High levels of fluoxetine reduce every step associated with biohydrogen production, including decreasing concentrations of ammonia nitrogen and phosphate and having a significant negative effect on enzyme activity.