Co-cultivation of Streptomyces and microalgal cells as an efficient system for biodiesel production and bioflocculation formation

The phytohormone producing Streptomyces rosealbus MTTC 12,951 (S.R) and green microalga Chlorella vulgaris MSU-AGM 14 (C.V) were cultivated in co-culture system to evaluate exogenous hormonal activity. Biosynthesis of indole-3-acetic acid (IAA) and their precursors were quantitatively evaluated by employing High Performance Liquid Chromatography (HPLC). The concentration of IAA (0.72 +/- 0.02 mu g mL(-1)) was observed to be elevated in co-cultivation system due to symbiotic interaction between Streptomyces and microalgae. In exchange, microalgae produced adequate volume of tryptophan (Trp) to induce IAA biosynthesis. The Trp stress in late exponential phase encouraged lipid accumulation (175 +/- 10 mg g(-1)). The bioflocculation property of microalgae ensures potential and economic viable harvesting process by reducing 148% input energy compared to conventional method. The overall results evidenced that C.V co-cultivation with S.R exhibits promotional behavior and serves as a promising cultivation process for microalgae in terms of cost efficiency and energy conservation.

Automated summary

In a co-culture system of Streptomyces and microalgae, the elevated concentration of IAA was observed due to symbiotic interaction between the two. Microalgae produced tryptophan to induce IAA biosynthesis and lipid accumulation. The bioflocculation property of microalgae reduces energy input and ensures cost efficiency in harvesting.