Production of polyhydroxyalkanoates from propylene oxide saponification wastewater residual sludge using volatile fatty acids and bacterial community succession

The active sludge treating propylene oxide saponification wastewater has heavy salt concentration and is hard to treat. The integration of the residual sludge treatment with polyhydroxyalkanoates (PHA) production may provide an economic and environment friendly solution. PHA production was therefore studied in two sequencing biological reactors with effective volume of 30 L using the active sludge. The two reactors, named as SBR-I and SBR-II, were fed with acetic acid, and a mixture of acetic acid and propionic acid respectively. PHA was obtained with a yield of 9.257 g/L in SBR-II. Also, the proportion of 3-hydroxyvalarate was enhanced from 5% to 30% in comparison to SBR-I (5.471 g/L). Illumina MiSeq and Pacific Biosciences sequencing platforms were used to evaluate the community structure, which revealed that the bacterial genera showed a high degree of diversity in the PHA accumulating microbial community. Azoarcus was the most dominant PHA accumulating microorganism after acclimation.

Automated summary

The study showed that integrating residual sludge treatment with PHA production can provide an economic and environmentally friendly solution for treating propylene oxide saponification wastewater. Results revealed that high PHA yield was obtained in SBR-II using active sludge, with a higher proportion of 3-hydroxyvalarate. Additionally, bacterial genera in the PHA accumulating microbial community showed high diversity, with Azoarcus being the dominant PHA accumulating microorganism after acclimation.