Green Roccella phycopsis Ach. mediated silver nanoparticles: synthesis, characterization, phenolic content, antioxidant, antibacterial and anti-acetylcholinesterase capacities

In this study, we develop here for the first time an easy, eco-friendly method for synthesizing silver nanoparticles (AgNPs) using the lichen Roccella phycopsis. AgNPs formation was first determined by a color change of the lichen filtrate to brown, subsequent to addition of AgNO3 solution, and confirmed by a maximum absorbance peak at 425 nm in UV-vis spectrum. Scanning electron microscope images showed a spherical shape with a size distribution between 11 and 18 nm, while the elemental composition was elucidated by the energy dispersive X-ray spectroscopy. The chemical compounds responsible for reduction and stabilization of silver nanoparticles were detected by Frourier transform infrared spectroscopy analysis. The synthesized R. phycopsis silver nanoparticles displayed a strong antioxidant activity. Further, the antibacterial activity was more effective against Gram-negative than Gram-positive bacteria. Besides, the R. phycopsis-AgNPs were potent in inhibiting acetylcholinesterase enzyme with IC50 value of 1.65 +/- 0.07 mg/mL.

Automated summary

A simple and eco-friendly method for synthesizing silver nanoparticles using the lichen Roccella phycopsis was developed in this study. The synthesized AgNPs demonstrated strong antioxidant and antibacterial activities, especially against Gram-negative bacteria. Additionally, the silver nanoparticles showed potent inhibition of acetylcholinesterase enzyme.