Isolation and characterization of a newly identified Clostridium butyricum strain SCUT343-4 for 1,3-propanediol production

A novel 1,3-propanediol (1,3-PDO) producing strain was isolated and identified as Clostridium butyricum with respect to its morphological and physiological characteristics, as well as 16S rDNA. The results of substrates test and stress tolerance indicated that C. butyricum SCUT343-4 could produce 1,3-PDO efficiently from glycerol. The optimal fermentation conditions were determined to be 5 g/L yeast extract at 37 degrees C and pH 6.5. To fully evaluate its 1,3-PDO production capacity, different cultivation strategies have been implemented. The highest 1,3-PDO concentration obtained for batch and fed-batch fermentation were 51.64 and 61.30 g/L, respectively. Immobilized cell fermentation in fibrous-bed bioreactor was also performed, and the concentration of 1,3-PDO further increased to 86 g/L with a yield of 0.52 g/g. In addition, the 1,3-PDO productivity reached 4.20 g/L h, which is the highest level reported for C. butyricum, demonstrating the potential of C. butyricum SCUT343-4 for 1,3-PDO production from glycerol.

Automated summary

A novel strain of Clostridium butyricum, SCUT343-4, has been identified as a potential producer of 1,3-PDO, showing high efficiency in production under optimized fermentation conditions and different cultivation strategies. The immobilized cell fermentation in fibrous-bed bioreactor resulted in the highest 1,3-PDO concentration and productivity reported for C. butyricum, indicating its great potential for 1,3-PDO production from glycerol.