1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the H-1, C-13 and N-15 chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.

Automated summary

The stem-loop SL1 is a conserved structural element in the SARS-CoV-2 RNA genome, present in both genomic and subgenomic mRNA species. It is a ubiquitous cis-regulatory RNA with potential functions throughout the viral life cycle. The chemical shift assignment of RNA construct 5_SL1 provides insight into the stability of the native SL1 structure.