期刊
BIOMEDICAL MATERIALS
卷 16, 期 5, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1748-605X/ac0d94
关键词
bovine cancellous bone; cellular and cytoskeletal morphology; cellular differentiation; low immunogenicity; bone regeneration
资金
- National Natural Science Foundation of China [51773123]
Xenogeneic bone has good biological activity, but overcoming immunogenicity while maintaining osteogenic abilities is challenging. By combining xenogeneic bone with poly amino acid (PAA), a new type of composite with bionics and low immunogenicity was obtained. Three composites of delipidized cancellous bone powder (DCBP) and PAA were designed and prepared by different methods, with DCBP/PAA EM showing the best cell proliferation and osteogenic differentiation in vitro.
Xenogeneic bone has good biological activity, but eliminating immunogenicity, while retaining osteogenic abilities, is a challenge. By combining xenogeneic bone with poly amino acid (PAA) that has an amide bond structure, a new type of composite conforming to bionics and low immunogenicity may be obtained. In this study, according to the principles of component bionics, three composites of delipidized cancellous bone powder (DCBP) and PAA were designed and obtained by an in situ polycondensation method, an extrusion molding (EM) method, and a solution-blend method. The three composites were all macroscopically uniform, non-cytotoxic, and demonstrated low immunogenicity by effective removal of residual antigens during preparation. Compared with PAA, mouse bone marrow mesenchymal stem cells (BMSCs) on the surfaces of three composites showed different cellular morphologies. The effects of different preparation methods and cellular morphology on cellular differentiation were confirmed by alkaline phosphatase activity, calcium nodule formation and the expression levels of osteogenic differentiation-related genes (bone morphogenetic protein 2, runt-related transcription factor 2, osteopontin and osteocalcin). Among these composites, DCBP/PAA EM showed best cell proliferation and osteogenic differentiation in vitro, and possessed greater bone formation than PAA in a rabbit femoral condyle study. This study may provide a new method for preparing bioactive bone repair materials with low immunogenicity and superior ability to stimulate differentiation of BMSCs in vitro and osteogenesis in vivo. DCBP/PAA EM might be a promising bone repair material for bone defect treatment.
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