期刊
BIOMACROMOLECULES
卷 22, 期 7, 页码 3138-3147出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.1c00619
关键词
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资金
- Yonsei University Futureleading Research Initiative of 2016 [2016-22-0054]
- Brain Korea 21(BK21) FOUR program
- Graduate School of YONSEI University Research Scholarship Grants in 2020
The study investigates the efficiency of anisotropically functionalized DNA nanostructures as biological signal inducers in 3D cell cultivation, with a focus on controlling cell adhesion capability. The results demonstrate significantly enhanced cell proliferation and homogeneous distribution of encapsulated cells within hydrogel matrices, showing promise for advancing conventional 3D culture platforms.
The development of supramolecular hydrogel scaffolds for the precise positioning of biochemical cues is paramount for applications such as tissue engineering. Nucleic acid engineering allows fabrication of three-dimensional (3D) nanostructures with high variability and nanoscale precision. In this study, aptamers were anisotropically functionalized onto branched DNA nanostructures to control their cell adhesion capability, and their efficiency as biological signal inducers for 3D cell cultivation was investigated. Each arm of the X-shaped DNA nanostructure (X-DNA) was functionalized with photo-cross-linkable or cell adhesion moieties, and the steric hindrance of the 3D DNA nanostructures on a cell was optimized. X-DNA nanostructures with cell-positioning parameters were rapidly photopolymerized to form hybrid hydrogels, and their effects on cell behaviors and positions were investigated. We observed that aptamer-functionalized X-DNA nanostructures exhibited significantly enhanced cell proliferation and provided homogeneous distribution and target-specific adhesion of encapsulated cells within hydrogel matrices. Overall, the anisotropic functionalization of DNA nanostructures provides a controllable function for the advancement of conventional 3D culture platforms.
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