期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 200, 期 4, 页码 1531-1537出版社
SPRINGERNATURE
DOI: 10.1007/s12011-021-02778-z
关键词
Kashin-Beck disease; Chondrocyte; Selenium; Carbohydrate chain; Lectin microarray
资金
- Shaanxi Natural Science Basic Research Projects [2021JM-037, 2019JM-486]
- National Natural Science Foundation of China key international cooperation project [81620108026]
This study found that adding selenium can affect the glycosylation level of chondrocyte glycoproteins in KBD patients, thereby influencing the pathogenesis of the disease.
In this study, we aimed to explore the effectiveness of selenium on the chondrocyte glycoprotein glycosylation which plays important roles in the pathogenesis of Kashin-Beck disease (KBD). Cartilage samples were collected from KBD patients after total knee replacement surgery. Chondrocytes were cultured with sodium selenium. The group of chondrocytes which were cultured without adding sodium selenium was considered as control group. Lectin microarray was used to screen the differences in lectin levels between KBD and KBD with selenium groups. Stronger signals for Bandeiraea simplicifolia (BS-I), Hippeastrum hybrid lectin (HHL), Pisum sativum agglutinin (PSA), Psophocarpus tetragonolobus lectin I (PTL-I), Psophocarpus tetragonolobus lectin II (PTL-II), Sophora japonica agglutinin (SJA), Lotus tetragonolobus lectin (LTL), and Triticum vulgaris (WGA) were observed in the KBD group. Meanwhile, Aleuria aurantia lectin (AAL), Lens culinaris agglutinin (LCA), Lycopersicon esculentum (tomato) lectin (LEL), Peanut agglutinin (PNA), and Sambucus nigra lectin (SNA) signals were lower in the KBD group. Selenium may have the function of influence the expression levels of carbohydrate chains Gal alpha 1,3-Gal, high mannose, and GlcNAc.
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