期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 200, 期 4, 页码 1883-1891出版社
SPRINGERNATURE
DOI: 10.1007/s12011-021-02760-9
关键词
Bombyx mori ovarian cell line; Oxidative damage; Titanium dioxide nanoparticles
资金
- China Agriculture Research System [CARS-18-ZJ0106]
- National Natural Science Foundation of China [31870148]
- China Postdoctoral Science Foundation [2019M651951]
- Science & Technology Support Program of Suzhou [SNG201925, SNG201912]
- QingLan Project of Jiangsu Province - Priority Academic Program Development of Jiangsu Higher Education Institutions
The research found that TiO2 NPs have toxic effects on the viability of BmN cells, with significant toxicity observed above 21 mg/L; Exposure to TiO2 NPs leads to ROS accumulation and DNA damage; Exposure to TiO2 NPs reduces the transcription of antioxidant genes, accumulates peroxides, and induces inflammation.
As a new type of biologically compatible material, TiO2 NPs are widely used in the industry as additives, drug carriers, and components of skin care products. Due to their wide use, residual TiO2 NPs in the environment are a safety concern that has attracted extensive attention. In this study, the ovarian cell line BmN of the model organism Bombyx mori was used to reveal the damaging effects of TiO2 NPs exposure. The results demonstrated that TiO2 NPs exhibited a dose-dependent effect on the relative cell viability, with significant toxic effects being observed above 20 mg/L. Oxidative damage analysis showed that ROS accumulated significantly in BmN cells after exposure to TiO2 NPs (P <= 0.05) and induced DNA damage. Further analysis revealed that the transcriptional levels of key superoxide dismutase genes (SOD) decreased significantly, while the transcriptions of key genes of the MAPK/NF-kappa B signaling pathway (P38, MEK, ERK and REL) and the downstream inflammatory factor genes (IL6 and TNFSF5) were all significantly up-regulated (P <= 0.05). Overall, our results indicate that exposure to TiO2 NPs leads to reduced transcription of antioxidant genes, accumulation of peroxides, and inflammation. These findings provide valuable data for the safety evaluation of environmental residues of TiO2 NPs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据