期刊
BIOFOULING
卷 37, 期 5, 页码 521-537出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2021.1933960
关键词
Catheter-associated urinary tract infections; Pseudomonas aeruginosa; quorum sensing; antivirulence; eugenol; biofilm
资金
- Indian Council of Medical Research, New Delhi [80/903/2014-ECD-1]
The study developed silicone segments coated with an antivirulence agent, eugenol, which effectively prevented biofilm formation and bacterial adhesion by inhibiting virulence gene expression. Drug release profiles and toxicity analysis confirmed the effectiveness and safety of the eugenol-coated segments.
The incidence of biofilm-linked catheter-associated urinary tract infections (CAUTIs) is increasing across the world. However, there is no clinical evidence to support the modifications of biomaterials, such as antimicrobial agent-coated catheters, that are known to reduce the risk of bacterial colonization and resistance development. The present study developed and tested silicone segments coated with an antivirulence agent, eugenol. The parameters for sol-gel preparation and coating were tailored to achieve a prolonged release of eugenol (for >35 days) at predefined antivirulence doses from dip-coated thin films. The eugenol-coated segments could prevent biofilm formation by Pseudomonas aeruginosa PAO1 as well as bacterial adhesion. Significant repression in the expression of virulence and biofilm-associated genes were recorded, confirming the antivirulence and biofilm inhibition properties of silicone segments coated with eugenol. The drug release profiles, efficacy analysis, neutrophil-response studies, and in vitro toxicity profiling further supported the contention that the activity of the eugenol-coated sections was effective and safe.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据