期刊
BIOCONTROL SCIENCE AND TECHNOLOGY
卷 31, 期 11, 页码 1231-1247出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2021.1940864
关键词
Phospholipase A; T; harzianum; T; atroviride; antagonism; S; rolfsii; biocontrol
资金
- National University of Cordoba - Argentina
Trichoderma harzianum showed the highest potential in phospholipase A activity, especially when the culture medium included the plant pathogenic fungus Sclerotium rolfsii. Additionally, both T. harzianum and T. atroviride exhibited higher activity in the first days of culture.
The aim of this work was to study the phospholipase A (PLA) enzyme activity of extracts from Trichoderma harzianum 1A and Trichoderma atroviride alpha Cp8 culture media. Fermentation on submerged culture was performed and PLA activity of extracts was evaluated as a function of culture time. A turbidimetric method for continuous analysis of phospholipase activity was applied. By this, vesicles rather than soluble substrates are used resembling the in vivo environment. In Trichoderma's culture experiments, lecithin phospholipids as inductors promoted a PLA expression level of the same order to the corresponding strain control assay. T. harzianum developed the highest PLA activity and was considered as the most potent strain. Furthermore, the activity level was clearly greater when the culture medium included the plant pathogenic fungus Sclerotium rolfsii. The T. harzianum strain showed the highest PLA potential. Besides, both T. harzianum and T. atroviride, developed higher PLA activity at the first days of culture. In the present work, T. harzianum 1A and T. atroviride alpha Cp8 at a lower level, showed an important PLA potential for S. rolfsii control. On the other side, a lower relative response was observed when both Trichoderma strains were assayed against other pathogens such as Macrophomina phaseolina and Rhizoctonia solani. The present work contributes to the scientific knowledge about PLA activity in Trichoderma and could be useful for technological purposes to prepare formulations for the biological control of S. rolfsii in agriculture.
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