期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1868, 期 7, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2021.119034
关键词
Astrocyte; Caloric restriction; Calcium signalling; Gap-junctions
资金
- Russian Science Foundation [20-14-00241]
- Russian Science Foundation [20-14-00241] Funding Source: Russian Science Foundation
The study observed alterations in the spatiotemporal properties of Ca2+ signals in protoplasmic astrocytes in the CA1 stratum radiatum of hippocampal slices from mice exposed to a caloric restriction (CR) diet. The changes included shorter duration and smaller size of Ca2+ events, reduced velocity of expansion and shrinkage, higher amplitude, faster rise and decay rates. These alterations were attributed to CR-induced morphological remodelling of astrocytes.
We analysed spatiotemporal properties of Ca2+ signals in protoplasmic astrocytes in the CA1 stratum radiatum of hippocampal slices from young (2-3 months old) mice housed in control conditions or exposed to a caloric restriction (CR) diet for one month. The astrocytic Ca2+ events became shorter in duration and smaller in size; they also demonstrated reduced velocity of expansion and shrinkage following CR. At the same time, Ca2+ signals in the astrocytes from the CR animals demonstrated higher amplitude and the faster rise and decay rates. These changes can be attributed to CR-induced morphological remodelling and uncoupling of astrocytes described in our previous study. CR-induced changes in the parameters of Ca2+ activity were partially reversed by inhibition of gap junctions/hemichannels with carbenoxolone (CBX). The effect of CBX on Ca2+ activity in CR-animals was unexpected because the diet already decreases gap junctional coupling in astrocytic syncytia. It may reflect the blockade of hemichannels also sensitive to this drug. Thus, CR-induced morphological remodelling of astrocytes is at least partly responsible for changes in the pattern of Ca2+ activity in the astrocytic network. How such changes in spatiotemporal Ca2+ landscape can translate into astrocytic physiology and neuron-glia interactions remains a matter for future studies.
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