期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1866, 期 9, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbalip.2021.158989
关键词
Lipid transport; Intracellular trafficking; Cholesterol analogues; Fluorescence microscopy; Organelle contact sites
资金
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [278001972 - TRR186]
- Moorfields Eye Charity [GR001004]
This review provides an overview of methods used to study subcellular cholesterol localization and transport routes, including those based on downstream effects like esterification and the use of cholesterol-binding molecules for visualization and quantification. Researchers can choose the most relevant approach based on the challenges and advantages highlighted for each method.
Cholesterol is an essential component of eukaryotic cellular membranes. Information about its subcellular localization and transport pathways inside cells are key for the understanding and treatment of cholesterol-related diseases. In this review we give an overview over the most commonly used methods that contributed to our current understanding of subcellular cholesterol localization and transport routes. First, we discuss methods that provide insights into cholesterol metabolism based on readouts of downstream effects such as esterification. Subsequently, we focus on the use of cholesterol-binding molecules as probes that facilitate visualization and quantification of sterols inside of cells. Finally, we explore different analogues of cholesterol which, when taken up by living cells, are integrated and transported in a similar fashion as endogenous sterols. Taken together, we highlight the challenges and advantages of each method such that researchers studying aspects of cholesterol transport may choose the most pertinent approach for their problem.
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