期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1865, 期 6, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2021.129869
关键词
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资金
- Swiss National Science Foundation [31003A-160038, 310030-189083]
- State Scholarship Fund of China Scholarship Council, Ministry of Education of the People's Republic of China
- Swiss National Science Foundation (SNF) [310030_189083, 31003A_160038] Funding Source: Swiss National Science Foundation (SNF)
The use of a Cas13-mediated conditional knockdown system can achieve the suppression of target proteins restricted to Purkinje cells in mixed cerebellar cultures.
Specific gene knockdown in cerebellar Purkinje cells is a major challenge because Purkinje cells only make up a small fraction of the cerebellum and off-target effects of shRNA occur. Little is known about the application of CRISPR-Cas13 and the resulting protein expression levels in Purkinje cells, a type of developing postmitotic neuron. We explored the possibility of a Cas13-mediated conditional knockdown system for cerebellar Purkinje cells. This system can achieve a suppression of the target proteins restricted to Purkinje cells in mixed cerebellar cultures.
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