期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
卷 1864, 期 9, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbagrm.2021.194732
关键词
Early brain; Development; Gene expression; MicroRNA; Nutrition; Honeybee
资金
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [CBB - APQ-02134-14]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2005/03926-5, 2011/03171-5]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [406734/2018-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Brasil (CAPES) [001]
- FAPEMIG
- FINEP Financiadora de Estudos e Projetos [PROINFRA 01/2008]
Differential brain morphogenesis in female honey bees is a key feature of caste development, influenced by differential feeding regimes in early larval stages. The study identified 53 differentially expressed genes (DEGs) in the brains of queens and workers, with miRNAs potentially involved in regulating these DEGs. Experimental confirmation of miRNA interactions with specific genes suggests a regulatory network governing early brain diphenism in honey bees.
Brain differential morphogenesis in females is one of the major phenotypic manifestations of caste development in honey bees. Brain diphenism appears at the fourth larval phase as a result of the differential feeding regime developing females are submitted during early phases of larval development. Here, we used a forward genetics approach to test the early brain molecular response to differential feeding leading to the brain diphenism observed at later developmental phases. Using RNA sequencing analysis, we identified 53 differentially expressed genes (DEGs) between the brains of queens and workers at the third larval phase. Since miRNAs have been suggested to play a role in caste differentiation after horizontal and vertical transmission, we tested their potential participation in regulating the DEGs. The miRNA-mRNA interaction network, including the DEGs and the royal- and worker-jelly enriched miRNA populations, revealed a subset of miRNAs potentially involved in regulating the expression of DEGs. The interaction of miR-34, miR-210, and miR-317 with Takeout, Neurotrophin1, Forked, and Masquerade genes was experimentally confirmed using a luciferase reporter system. Taken together, our results reconstruct the regulatory network that governs the development of the early brain diphenism in honey bees.
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