Protein-fragment complementation assays for large-scale analysis of protein-protein interactions

Protein-protein interactions (PPIs) orchestrate nearly all biological processes. They are also considered attractive drug targets for treating many human diseases, including cancers and neurodegenerative disorders. Protein-fragment complementation assays (PCAs) provide a direct and straightforward way to study PPIs in living cells or multicellular organisms. Importantly, PCAs can be used to detect the interaction of proteins expressed at endogenous levels in their native cellular environment. In this review, we present the principle of PCAs and discuss some of their advantages and limitations. We describe their application in large-scale experiments to investigate PPI networks and to screen or profile PPI targeting compounds.

Automated summary

Protein-protein interactions play a crucial role in biological processes and are attractive drug targets for various human diseases. PCAs offer a direct and versatile approach to study PPIs, enabling the detection of interactions in their native cellular environment. They are valuable tools for large-scale experiments exploring PPI networks and drug screening.