期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 557, 期 -, 页码 174-179出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.03.146
关键词
Osteocalcin; GPR158; GPRC6A; PC12
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [17K11649, 18K09510, 18K09521, 17K19766, 17H01595, 20H03854]
- Grants-in-Aid for Scientific Research [18K09510, 17K11649, 18K09521, 17K19766, 20H03854] Funding Source: KAKEN
Bone matrix protein osteocalcin (OC) is involved in the development of learning and memory, and the prevention of anxiety-like behaviors in mice. This study found that OC can enhance cell proliferation, differentiation, and survival in neurons by binding to its candidate receptor GPR158.
Involvement of the bone matrix protein osteocalcin (OC) in the development of learning and memory, and the prevention of anxiety-like behaviors in mice. However, the direct effects of OC on neurons are still unknown comparing to the mechanism how OC affects systemic energy expenditure and glucose homeostasis. In this study, we investigated the effect of OC on proliferation, differentiation, and survival of neurons using the rat pheochromocytoma cell line PC12. RT-PCR analysis for OC receptor candidates revealed that Gpr158, but not Gprc6a, mRNA was expressed in PC12 cells. The growth of PC12 cells cultured in the presence of 5-50 ng/mL of either uncarboxylated (GluOC) or carboxylated (GlaOC) OC was increased compared to cells cultured in the absence of OC. In addition, NGF-induced neurite outgrowth was enhanced by OC, and H2O2-induced cell death was sup-pressed by pretreatment with OC. All of these results were observed for both GluOC and GlaOC at comparable levels, suggesting that OC may directly affect cell proliferation, differentiation, and survival by binding to its candidate receptor, GPR158. (C) 2021 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据