期刊
EXPERIMENTAL CELL RESEARCH
卷 342, 期 1, 页码 20-31出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2016.03.001
关键词
Ubc9; SUMOylation; KLF4; VSMC; Proliferation
资金
- National Natural Science Foundation of People's Republic of China [31271224, 31271396, 31301136]
- Fok Ying Tung Education Foundation [131037]
- National Basic Research Program of China [2012CB518601]
- Hebei key Scientific and Technological Project [13967607D]
The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue due to its major implications for the prevention of pathological vascular conditions. The objective of this work was to assess the function of small ubiquitin-like modifier (SUMO)ylated Kruppel-like transcription factor 4 (KLF4) in the regulation of VSMC proliferation in cultured cells and in animal models with balloon injury. We found that under basal conditions, binding of non-SUMOylated KLF4 to p300 activated p21 (p21(WAF1/CIP1))transcription, leading to VSMC growth arrest. PDGF-BB promoted the interaction between Ubc9 and KLF4 and the SUMOylation of KLF4, which in turn recruited transcriptional corepressors to the p21 promoter. The reduction in p21 enhanced VSMC proliferation. Additionally, the SUMOylated KLF4 did not affect the expression of KLF4, thereby forming a positive feedback loop enhancing cell proliferation. These results demonstrated that SUMOylated KLF4 plays an important role in cell proliferation by reversing the transactivation action of KLF4 on p21 induced with PDGF-BB. (C) 2016 Elsevier Inc. All rights reserved.
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