期刊
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
卷 68, 期 4, 页码 223-231出版社
ELSEVIER GMBH
DOI: 10.1016/j.etp.2015.12.004
关键词
Atrazine; Lycopene; Cytochrome P450s; Hepatic microsome; Transcriptional regulation
资金
- Program for New Century Excellent Talents in University [NECT-1207-02]
- China Postdoctoral Science Foundation [2012T50301]
- Academic Backbone Project of Northeast Agricultural University [15XG16]
- Application Technology Research and Development Projects of Heilongjiang Province [GA14B105]
- Special Project of International Technology Cooperation and Exchanges [2013DFG62260]
Atrazine (ATR) is primarily distributed in liver and hazardous to animal health. Cytochrome P450 enzyme system (CYP450s) is responsible for the biotransformation of toxic substances. Lycopene (LYC) prevents the herbicide-induced toxicity. However, it is unclear that LYC protects against ATR-induced hepatotoxicity via modifying CYP450s. To ascertain the chemoprevention of LYC on ATR-induced hepatotoxicity, male Kunming mice were treated with LYC (5 mg/kg) and/or ATR (50 mg/kg or 200 mg/kg) by gavage administration for 21 days. These results showed that ATR induced the increase of total CYP450 and Cytochrome b5 (Cyt b5) contents and stimulated the activities of CYP450s enzymes (erythromycin N-demethylase (ERND), aminopyrin N-demethylase (APND), aniline-4-hydeoxylase (AH) and NADPH-cytochrome c reductase (NCR)) in hepatic microsomes. The mRNA expressions of six CYP450s genes (increase: CYP1a1, CYP2a4, CYP3a57 and decrease: CYP2f2, CYP3a11, CYP4a31) were significantly influenced by ATR. LYC modulated the contents and activities of CYP450s and normalized the expressions of four CYP450s genes (CYP1b1, CYP2a4, CYP2e1, and 4A14). These findings suggested that ATR induced hepatic CYP450s disturbance and influenced the gene expression of CYP450s. Lycopene protected against hepatic CYP450s disturbance induced by ATR via modifying the hepatic CYP450s activities and transcription in mice. (C) 2015 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据