Ameliorative effect of propolis on the cadmium-induced reproductive toxicity in male albino rats

Propolis is a potent antioxidant and a free radical scavenger. The present study aimed to investigate protective effects of propolis extract on cadmium-induced testicular damage, apoptosis, HIF-1 alpha expression and toxicity in rat's testis tissue. A total of 32 male rats were equally divided into four study groups namely, control, Cd (1 mg/kg/day), Cd + propolis (50 mg/kg/day) and propolis. The rats were decapitated under ketamine anesthesia and their testes tissues were removed. Serum testosterone, tissue malondialdehyde and HIF-1 alpha levels, HIF-1 alpha expression, apoptosis and histopathological damage scores were then compared. In the Cd group, the diameters of seminiferous tubules, tubular biopsy score of Johnsen and serum testosterone levels were decreased compared control group, but tissue HIF-1 alpha and tissue MDA levels was higher than control group. The immunoreactivity of HIF-1 alpha and the number of apoptotic cells were increased in Cd group. Further-more, the propolis treated group showed an improved histological appearance in the Cd group. Thus, the results suggest that propolis acts as a potent protective agent against Cd-induced testicular toxicity in rats. (C) 2016 Elsevier Inc. All rights reserved.