Molecular characterization of a putative new cavemovirus isolated from wild chicory (Cichorium intybus)

A putative new virus with sequence similarity to members of the genus Cavemovirus in the family Caulimoviridae was identified in wild chicory (Cichorium intybus) by next-generation sequencing (NGS). The putative new virus was tentatively named chicory mosaic cavemovirus (ChiMV), and its genome was determined to be 7,775 nucleotides (nt) long with the typical genome organization of cavemoviruses. ORF1 encodes a putative coat protein/movement polyprotein (1,278 aa), ORF2 encodes a putative replicase (650 aa), and ORF3 encodes a putative transactivator factor (384 aa). The first two putative proteins have 46.2% and 68.7% amino acid sequence identity to the CP/MP protein (YP_004347414) and replicase (YP_004347415), respectively, of sweet potato collusive virus (SPCV). ORF3 encodes a protein with 38.5% amino acid sequence identity to the putative transactivator factor (NP_056849) of cassava vein mosaic virus (CsVMV). The new putative viral genome and those of three cavemoviruses (epiphyllum virus 4 [EpV-4], SPCV, and CsVMV) differ by 24-27% in the nt sequence of the replicase gene, which exceeds the species demarcation cutoff (>20%) for the family.

Automated summary

A putative new virus, tentatively named chiory mosaic cavemovirus (ChiMV), with sequence similarity to members of the genus Cavemovirus in the family Caulimoviridae, was identified in wild chicory by next-generation sequencing. The genome of ChiMV was determined to be 7,775 nucleotides long with the typical genome organization of cavemoviruses. Protein sequences encoded by ChiMV showed varying degrees of amino acid sequence identity to known viruses in the same family.