Dental tissue-derived stem cell sheet biotechnology for periodontal tissue regeneration: A systematic review

Objective: This study aimed to conduct a systematic review of the use of a cell sheet formed by mesenchymal stem cells derived from dental tissues (ddMSCs) for periodontal tissue regeneration in animal models in comparison with any other type of regenerative treatment. Design: PubMed and Scopus databases were searched for relevant studies up to December 2020. The review was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-analysis guidelines. Results: Of the 1542 potentially relevant articles initially identified, 33 fulfilled the eligibility criteria and were considered for this review. Even with a wide variety of selected study methods, the periodontal tissue was always regenerated; this indicates the potential for the use of these cell sheets in the future of periodontics. However, this regeneration process is not always complete. Conclusion: Despite the implantation, ddMSCs sheets have a great potential to be used in the regeneration of periodontal tissue. More in vivo studies should be conducted using standardized techniques for cell sheet im-plantation to obtain more robust evidence of the relevance of using this modality of cell therapy for periodontal tissue regeneration.

Automated summary

The review of 33 studies indicates that cell sheets formed by mesenchymal stem cells derived from dental tissues have great potential for periodontal tissue regeneration in animal models, but the regeneration process is not always complete.