Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment

In this study, we report a novel Gram-positive bacterium, designated as strain CS13(T), isolated from deep-sea sediment collected in the cold seep area of the South China Sea. Growth of strain CS13(T) occurred at 16-37 degrees C (optimum 25-28 degrees C), pH 7.0-9.0 (optimum, 7.0), and 0-8% (w/v) NaCl (optimum, 2-3%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CS13(T) belonged to the genus Bacillus. The closest phylogenetic neighbors of strain CS13(T) are Bacillus carboniphilus JCM 9731(T) (96.0%), Bacillus pakistanensis NCCP-168(T) (95.7%) and Bacillus acidicola 105-2(T) (95.6%). The genomic DNA G + C content of strain CS13(T) is 43.7 mol%. The principal respiratory quinone was menaquinone 7 (MK-7). The polar lipids of CS13(T) contained diphosphatidylglycerol, phosphatidylglycerol, phospholipid, and glycolipid. The major fatty acids of CS13(T) contained anteiso-C-15:0, anteiso-C-17:0, C-16:0 and C-18:0. Strain CS13(T) harboured meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic, physiological, biochemical, and morphological analyses suggested that strain CS13(T) represents a novel species of genus Bacillus, and the name Bacillus fonticola sp. nov. is proposed for the type species CS13(T) (= CCTCC AB 2019194(T) = JCM 33663(T)).

Automated summary

A novel Gram-positive bacterium, Bacillus fonticola sp. nov., designated as strain CS13(T), was isolated from deep-sea sediment in the cold seep area of the South China Sea. It belongs to the genus Bacillus and shows unique physiological, biochemical, and morphological characteristics compared to closely related species. Phylogenetic analysis suggests that strain CS13(T) represents a distinct species within the genus Bacillus.