期刊
AQUATIC TOXICOLOGY
卷 238, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2021.105913
关键词
Imidacloprid; Freshwater crayfish; Locomotion; Oxidative stress; Microbiota
资金
- Key Science Research Program of Liangshan [17NYCX0015]
- Shuanggao Science Research Program in Xichang University [LGZ201914, LGLZ201809]
- Project of Science and Technology Department of Sichuan Province [2020YJ0399]
The study found that IMI at environmentally realistic concentrations can cause AChE inhibition and oxidative stress in crayfish, leading to impaired locomotion. IMI also affects digestive functions by enzyme inhibition and dysbiosis of gut microbiota.
Imidacloprid (IMI) is used in integrated farming like the rice-crayfish co-culture system to prevent water weevil. However, the toxic effect of IMI on the freshwater crayfish Procambarus clarkii is unknown. In the current study, the effects of IMI on the locomotion, antioxidative status, digestion and intestinal microbiota of P. clarkii were investigated. The results showed that IMI caused locomotion impairment with reduced crawl velocity, and attenuated their dark preference, aggressiveness and reversal ability. Inhibited AChE in muscle and hepatopancreas indicates the neurotoxicity of IMI which may directly lead their locomotion dysfunction. The increase of antioxidative enzymes activity and MDA level were found after 25 mu g/L and 250 mu g/L exposure. Significant upregulation of several antioxidative and immune-related genes, including CZ-SOD, CAT, GPx, GST, AFL, proPO, HSP27 and HSP70 confirmed that oxidative stress was induced in all treatments when exposed to IMI. In addition, there was significant increase of LDH, indicating the different energy allocation during the exposure. Meanwhile, results from DNA damage analysis showed elevated OTM value and 8-OHdG level in hepatopancretic cells. On the other hand, decreases of alpha-amylase, lipase and increase of trypsin in hepatopancreas was observed at 25 and 250 mu g/L. In addition, significant changes of composition of intestinal microbiota at both phylum and genus levels were observed according to the 16S rRNA sequencing results. Increase of pathogenic genera and decrease of beneficial bacterial communities revealed the disequilibrium of intestinal flora of crayfish. In summary, results in the present study suggest that IMI at environmentally realistic concentration could induce AChE inhibition and oxidative stress, conjointly leading the locomotion impairment in crayfish. IMI also affected the digestive functions by enzymes inhibition and gut microbiota dysbiosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据