期刊
AQUATIC TOXICOLOGY
卷 236, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2021.105843
关键词
Endocrine disruption; Sexual differentiation; Gene transcription; RNA-Seq; Sunscreen chemicals
资金
- CUHK [4053363]
Studies have shown that Octocrylene (OC) has toxic effects on zebrafish and zebrafish liver cells, inducing gene expression and affecting hormone synthesis and antioxidant pathways. The findings support banning the use of OC in cosmetics.
Octocrylene (OC) is a broad-spectrum ultraviolet-absorbing chemical used in sunscreen and other personal care products. Its health effects are a concern because it has been detected in water, fish, humans, and food chains. In vivo and in vitro investigations were performed in zebrafish (Danio rerio) larvae and a zebrafish liver cell line (ZFL), respectively, to understand the potential risks and molecular mechanisms of OC toxicity. The 96-h median lethal concentration (LC50) of OC was determined to be 251.8 mu M in larvae and 5.5 mu M in ZFL cells. Quantitative real-time PCR (qRT-PCR) showed that OC induced the expression of genes for CYPs (CYP1A, CYP3A65), estrogen receptors (ER alpha, ER beta 1, GPER), vitellogenin (VTG1), and sex determination (BRCA2, CYP19A, DMRT1, SOX9A), both in vitro and in vivo. A whole-transcriptome sequencing method was used to evaluate the gene expression profile of larvae exposed to OC. OC was found to mediate the biosynthesis of estrogens (such as estriol) and affect the antioxidant pathway (glutathione transferases and peroxisome). These findings clarify the toxic effects and molecular mechanisms of OC and support banning its use in cosmetics.
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