4.5 Article

Transcriptome analysis reveals hub genes in the hepatopancreas of Exopalaemon carinicauda in response to hypoxia and reoxygenation

期刊

AQUACULTURE INTERNATIONAL
卷 29, 期 4, 页码 1785-1811

出版社

SPRINGER
DOI: 10.1007/s10499-021-00717-2

关键词

E; carinicauda; Hypoxia and reoxygenation; RNA-seq; WGCNA

资金

  1. Jiangsu Provincial Agricultural Major New Varieties Creation Project [PZCZ201747]
  2. fifth 226 Project cultivation fund support project of Nantong
  3. Science and Technology Plan Project of Nantong in 2019 [JC2019057]
  4. Jiangsu Provincial Agricultural Science and Technology Independent Innovation Project in 2018 [CX18-2010]

向作者/读者索取更多资源

This study investigated the molecular mechanisms underlying the adaptation of the ridgetail white prawn to hypoxia and reoxygenation through transcriptomic analysis. It identified key genes and pathways related to hypoxia and reoxygenation, shedding light on the biological responses of the prawn during these processes.
Hypoxia is a common stressor in shrimp, but the molecular mechanisms underlying the adaptation of the ridgetail white prawn, Exopalaemon carinicauda, to hypoxia and reoxygenation remain poorly understood. In the present study, ridgetail white prawns were exposed to gradual changes in hypoxia for 6 h via oxygen consumption in a static respiration chamber and then rapidly aerated to allow reoxygenation for 8 h. The dissolved oxygen concentration after 6 h of hypoxia was 1.04 mg/L, and the survival rate of the shrimp was 47%. A transcriptomic analysis of hepatopancreas tissues after 0 (control), 3 (hypoxic starting point), and 6 (hypoxic lethal half point) h of exposure to hypoxia and after 1 and 8 h of reoxygenation was conducted using the Illumina HiSeq(TM) 4000 high-throughput sequencing platform. A total of 93,227 genes were obtained, and 4315 of these genes were identified as differentially expressed genes (DEGs) that respond to hypoxia and reoxygenation. A KEGG pathway enrichment analysis suggested that all identified DEGs were mostly enriched in ribosome biogenesis in eukaryotes, apoptosis, the longevity regulating pathway, the MAPK signaling pathway, and protein processing in the endoplasmic reticulum. In addition, 20,203 genes with RPKM values >= 1 were parsed into 20 modules through a weighted gene coexpression network (WGCNA), and three of these modules were related to hypoxia and reoxygenation. GO and KEGG enrichment analyses of these three modules were then performed, and three hub genes were identified based on their connectivity: RREB1 (Ras-responsive element-binding protein 1-like), UBE1 (ubiquitin-activating enzyme E1), and an unknown gene. This study might help further elucidate the hypoxia tolerance mechanism of E. carinicauda.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据