期刊
APPLIED SURFACE SCIENCE
卷 556, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2021.149735
关键词
Hcy aptasensor; Homocysteine; Au NPs; GS; Electrochemistry; Biosensor
类别
资金
- Natural Science Foundation of Shanghai [18ZR1405000]
- National Natural Science Foundation of China [61874034, 51861135105, 11804055]
An electrochemical biosensor based on Hcy aptamer/Au nano-particles/graphene sponge was designed for the detection of homocysteine. The biosensor showed good selectivity and reproducibility in experimental results, with potential applications in the preliminary detection of various diseases associated with Hcy.
The increase of homocysteine (Hcy) is closely related with the development of many diseases. Nevertheless, the effective detection of Hcy remains challenging due to the lack of a simple, specific, and cost-efficient method. Herein, an electrochemical biosensor based on Hcy aptamer/Au nano-particles/graphene sponge (Aptamer/Au NPs/GS) was designed for the detection of homocysteine. The biosensor employed methylene blue (MB) as a redox indicator. Due to the change of aptamer conformation, the adsorbed MB was released from aptamer, resulting in the decrease of electrochemical response. In the range of 1-100 mu M, the concentration of Hcy had a good linear relationship with the relative redox peak current (Delta I) of MB and the lowest detection limit was 1 mu M. The experimental results demonstrated the aptasensor had good selectivity and reproducibility. In real serum sample, the aptasensor showed an acceptable recovery rate indicating its potential applications in the preliminary detection of various diseases associated with Hcy.
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