期刊
APPLIED SPECTROSCOPY
卷 75, 期 8, 页码 994-1002出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/00037028211025575
关键词
Ultraviolet resonance Raman; UVRR; flavin adenine dinucleotide; FAD; nicotinamide adenine dinucleotide; NADH; adenine; pH dependence; protonation
资金
- Ministry of Culture and Science of the state of North Rhine-Westfalia
- Senate Chancellery of the Federal State of Berlin
- Federal Ministry for Education and Research (BMBF)
- EFRE [1.8/07]
- DFG [Kn557/12-1, GSC 1013 SALSA]
The ultraviolet resonance Raman spectra of NAD+ and FAD in aqueous solution at physiological concentration were compared in order to distinguish them from their building block, adenine, in potential co-occurrence in biological materials. The measurement of spectra at different pH values showed characteristic changes mainly due to deprotonation of flavin and nicotinamide moieties, and protonation of adenine.
The ultraviolet resonance Raman spectra of the adenine-containing enzymatic redox cofactors nicotinamide adenine dinucleotide and flavin adenine dinucleotide in aqueous solution of physiological concentration are compared with the aim of distinguishing between them and their building block adenine in potential co-occurrence in biological materials. At an excitation wavelength of 266 nm, the spectra are dominated by the strong resonant contribution from adenine; nevertheless, bands assigned to vibrational modes of the nicotinamide and the flavin unit are found to appear at similar signal strength. Comparison of spectra measured at pH 7 with data obtained pH 10 and pH 3 shows characteristic changes when pH is increased or lowered, mainly due to deprotonation of the flavin and nicotinamide moieties, and protonation of the adenine, respectively.
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