Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats

Polyunsaturated fatty acids (PUFA)-derived bioactive lipid mediators called oxylipins have been shown to influence muscle growth, inflammation and repair in select muscles. Since individual oxylipins have varying effects and potencies, broad profiling in differing muscle types is required to further understand their overall effects. In addition, diet and sex are key determinants of oxylipin levels. Therefore, to provide comprehensive data on oxylipin profiles in rat soleus (SO), red gastrocnemius (RG), and white gastrocnemius (WG) muscles, female and male weanling Sprague-Dawley rats were provided control or experimental diets enriched in n-3 (omega-3) or n-6 (omega-6) PUFA for 6 weeks. Free oxylipin analysis by HPLC/MS/MS revealed that SO muscle had 25% more oxylipins and 4-13 times greater oxylipin mass than WG muscle. Dietary n-3 PUFA (alpha-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid) each increased n-3 oxylipins derived directly from their precursors and several that were not direct precursors, while reducing arachidonic acid derived oxylipins. Dietary linoleic acid had few effects on oxylipins. Oxylipins with a sex effect were higher in females in SO and RG. Oxylipins generally reflected the effects of diet and sex on PUFA, but there were exceptions. These fundamental oxylipin profile data provide groundwork knowledge and context for future research onmuscle oxylipin functions. Novelty: Rat SO compared with RG and WG muscles have a higher number and greater mass of oxylipins. Oxylipins generally reflect diet effects on PUFA in all muscles, but there are notable exceptions. fOxylipins in SO and RG are higher in females.

Automated summary

Polyunsaturated fatty acids (PUFA)-derived bioactive lipid mediators known as oxylipins have distinct effects on muscle growth, inflammation, and repair in different types of muscles, with sex and diet playing key roles in determining oxylipin levels. Analysis revealed that rat SO muscles had higher levels of oxylipins compared to RG and WG muscles, with n-3 PUFA increasing specific oxylipins and reducing others derived from arachidonic acid. Additionally, sex played a role in the levels of oxylipins in SO and RG muscles, with females showing higher levels.