期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 105, 期 19, 页码 7353-7365出版社
SPRINGER
DOI: 10.1007/s00253-021-11564-9
关键词
Telomerase reverse transcriptase; Fungal growth; Oxidative stress; ROS; Ganoderic acid
资金
- National Natural Science Foundation of China [31902090]
- Science and Technology Project of Henan Province [202102110019]
- High-level Talent Program of Henan Institute of Science and Technology [2018004]
This study investigates the non-canonical roles of telomerase reverse transcriptase (TERT) in Ganoderma lucidum, using RNA interference to silence the GlTert gene. The results show that GlTert plays a crucial role in fungal growth, oxidative stress response, and ganoderic acid (GA) biosynthesis in G. lucidum.
The telomerase reverse transcriptase (TERT) is the core catalytic subunit of telomerase. Its canonical function is synthesizing telomeric repeats to maintain telomere length and chromosomal stability. Accumulating evidence suggests that TERT has other important fundamental functions in addition to its catalytic telomere repeat synthesis activity. However, the non-canonical roles of TERT independent of its enzymatic activity are not clear in filamentous fungi. In the present study, we characterized the GlTert gene in Ganoderma lucidum. The non-canonical roles of GlTert were explored using GlTert-silenced strains (Terti8 and Terti25) obtained by RNA interference. Silencing GlTert delayed the fungal growth, decreased the length between hyphal branches, and induced fungal resistance to oxidative stress in G. ludicum. Further examination revealed that the intracellular ROS (reactive oxygen species) levels were increased while the enzyme activities of the antioxidant systems (superoxide dismutase, catalase, glutathione peroxidase, and ascorbate peroxidase) were decreased in GlTert-silenced strains. In addition, silencing GlTert decreased the ganoderic acid (GA) biosynthesis of G. lucidum. Taken together, our results indicate that GlTert plays a fundamental function on fungal growth, oxidative stress, and GA biosynthesis in G. lucidum, providing new insights for the canonical functions of TERT in filamentous fungi.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据