Streptomonospora litoralis sp. nov., a halophilic thiopeptides producer isolated from sand collected at Cuxhaven beach

Strain M2(T) was isolated from the beach of Cuxhaven, Wadden Sea, Germany, in course of a program to attain new producers of bioactive natural products. Strain M2(T) produces litoralimycin and sulfomycin-type thiopeptides. Bioinformatic analysis revealed a potential biosynthetic gene cluster encoding for the M2(T) thiopeptides. The strain is Gram-stain-positive, rod shaped, non-motile, spore forming, showing a yellow colony color and forms extensively branched substrate mycelium and aerial hyphae. Inferred from the 16S rRNA gene phylogeny strain M2(T) affiliates with the genus Streptomonospora. It shows 96.6% 16S rRNA gene sequence similarity to the type species Streptomonospora salina DSM 44593( T) and forms a distinct branch with Streptomonospora sediminis DSM 45723( T) with 97.0% 16S rRNA gene sequence similarity. Genome-based phylogenetic analysis revealed that M2(T) is closely related to Streptomonospora alba YIM 90003( T) with a digital DNA-DNA hybridisation (dDDH) value of 26.6%. The predominant menaquinones of M2(T) are MK-10(H-6), MK-10(H-8), and MK-11(H-6) (> 10%). Major cellular fatty acids are iso-C-16:0,C- anteiso C-17:0 and C-18:0 10-methyl. The polar lipid profile consisted of diphosphatidylglycerol phosphatidyl glycerol, phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine, three glycolipids, two unknown phospholipids, and two unknown lipids. The genome size of type strain M2(T) is 5,878,427 bp with 72.1 mol % G + C content. Based on the results obtained from phylogenetic and chemotaxonomic studies, strain M2(T) (= DSM 106425( T) = NCCB 100650( T)) is considered to represent a novel species within the genus Streptomonospora for which the name Streptomonospora litoralis sp. nov. is proposed.

Automated summary

Strain M2(T) was isolated from the beach of Cuxhaven, Wadden Sea, Germany, producing litoralimycin and sulfomycin-type thiopeptides. It belongs to the genus Streptomonospora and represents a novel species, Streptomonospora litoralis sp. nov., based on phylogenetic and chemotaxonomic studies.