期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 65, 期 10, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.01009-21
关键词
aminoglycoside resistance; 16S rRNA; methyltransferase
资金
- National Institutes of Health [R01AI104895, R21AI135522, R21AI151362]
Posttranslational methylation at position A1408 of 16S rRNA leads to pan-aminoglycoside resistance, encompassing both 4,5- and 4,6-disubstituted 2-deoxystreptamine (DOS) aminoglycosides. NpmB1 and NpmB2 are the second group of acquired pan-aminoglycoside resistance 16S rRNA methyltransferases, conferring resistance to all clinically relevant aminoglycosides, including 4,5-DOS agents. The encoding gene for NpmB1 was likely acquired by E. coli from a soil bacterium.
Posttranslational methylation of the A site of 16S rRNA at position A1408 leads to pan-aminoglycoside resistance encompassing both 4,5- and 4,6-disubstituted 2-deoxystreptamine (DOS) aminoglycosides. To date, NpmA is the only acquired enzyme with such a function. Here, we present the function and structure of NpmB1, whose sequence was identified in Escherichia coli genomes registered from the United Kingdom. NpmB1 possesses 40% amino acid identity with NpmA1 and confers resistance to all clinically relevant aminoglycosides, including 4,5-DOS agents. Phylogenetic analysis of NpmB1 and NpmB2, its single-amino-acid variant, revealed that the encoding gene was likely acquired by E. coli from a soil bacterium. The structure of NpmB1 suggests that it requires a structural change of the beta 6/7 linker in order to bind to 16S rRNA. These findings establish NpmB1 and NpmB2 as the second group of acquired pan-aminoglycoside resistance 16S rRNA methyltransferases.
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