期刊
ANIMAL BIOTECHNOLOGY
卷 34, 期 1, 页码 85-92出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2021.1935980
关键词
Major histocompatibility complex (MHC); microsatellite markers; goats; genetic diversity; selection
This study compared and analyzed the genetic diversity and population structure of the MHC-DQB1 gene exon 2 and 13 autosomal neutral microsatellite markers in 14 Chinese goat breeds, exploring the potential evolutionary mechanism of the MHC. The results showed that positive and balancing selection played a major role in maintaining the genetic diversity of DQB, and trans-species polymorphism of MHC in bovines was common.
This study compared and analyzed the genetic diversity and population structure of exon 2 of the DQB1 gene and 13 autosomal neutral microsatellite markers from 14 Chinese goat breeds to explore the potential evolutionary mechanism of the major histocompatibility complex (MHC). A total of 287 haplotypes were constructed from MHC-DQB1 exon 2 from 14 populations, and 82 nucleotide polymorphic sites (SNPs, 31.78%) and 172 heterozygous individuals (79.12%) were identified. The F-ST values of the microsatellites and MHC-DQB ranged between 0.01831-0.26907 and 0.00892-0.38871, respectively. Furthermore, 14 goat populations showed rich genetic diversity in the microsatellite loci and MHC-DQB1 exon 2. However, the population structure and phylogenetic relationship represented by the two markers were different. Positive selection and Tajima's D test results showed the occurrence of a diversified selection mechanism, which was primarily based on a positive and balancing selection in goat DQB. This study also found that the DQB sequences of bovines exhibited trans-species polymorphism (TSP) among species and families. In brief, this study indicated that positive and balancing selection played a major role in maintaining the genetic diversity of DQB, and TSP of MHC in bovines was common, which enhanced the understanding of the MHC evolution.
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