期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 50, 页码 26298-26302出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202108939
关键词
hyperpolarization; NMR spectroscopy; parahydrogen; RASER
资金
- NSF [CHE-1904780]
- National Cancer Institute [1R21CA220137]
- DOD CDMRP [W81XWH-15-1-0271, W81XWH-20-10576]
- NIBIB [R21EB025313, R01EB029829]
- NHLBI [R21 HL154032]
- North Carolina Biotechnology Center
- Mallinckrodt Foundation
This study demonstrates the utility of Radiofrequency Amplification by Stimulated Emission Radiation (RASER) for background-free proton detection of hyperpolarized biomolecules in a clinically relevant magnetic field. The results show that RASER detection without radio-frequency excitation could pave the way to new applications in high-resolution NMR spectroscopy and in vivo spectroscopy and imaging.
We report on the utility of Radiofrequency Amplification by Stimulated Emission Radiation (RASER) for background-free proton detection of hyperpolarized biomolecules. We performed hyperpolarization of approximate to 0.3 M ethyl acetate via pairwise parahydrogen addition to vinyl acetate. A proton NMR signal with signal-to-noise ratio exceeding 100 000 was detected without radio-frequency excitation at the clinically relevant magnetic field of 1.4 T using a standard (non-cryogenic) inductive detector with quality factor of Q=68. No proton background signal was observed from protonated solvent (methanol) or other added co-solvents such as ethanol, water or bovine serum. Moreover, we demonstrate RASER detection without radio-frequency excitation of a bolus of hyperpolarized contrast agent in biological fluid. Completely background-free proton detection of hyperpolarized contrast agents in biological media paves the way to new applications in the areas of high-resolution NMR spectroscopy and in vivo spectroscopy and imaging.
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