期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 35, 页码 19113-19116出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202107785
关键词
GABA; hydrolysis; natural products; NMR spectroscopy; picrotoxin
资金
- National Institute of Health [R35 GM122606]
- Jiangsu Industrial Technology Research Institute
The plant metabolite picrotoxinin (PXN) is commonly used in neuroscience to study GABAergic signaling. The hydrolysis of PXN in weakly alkaline media actually occurs at a closed lactone, contributing to a longstanding misunderstanding in the literature.
The plant metabolite picrotoxinin (PXN) is a widely used tool in neuroscience for the identification of GABAergic signaling. Its hydrolysis in weakly alkaline media has been observed for over a century and the structure of the unstable hydrolysis intermediate was assigned by analogy to the degradation product picrotoxic acid. Here we show this assignment to be in error and we revise the structure of the hydrolysis product by spectroscopic characterization in situ. Counterintuitively, hydrolysis occurs at a lactone that remains closed in the major isolable degradation product, which accounts for the longstanding mistake in the literature.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据