期刊
ANALYTICAL CHEMISTRY
卷 93, 期 30, 页码 10511-10518出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01453
关键词
-
资金
- Natural Science Foundation of China [21675046, 21735002, 21521063, 21806186, 21775036, 21874035]
In this study, a hand-in-hand in situ tile assembly was introduced for improved visualization of TK1 mRNA and killing of cancer cells through pH-responsive and aptamer-functionalized mechanisms. The research showed enhanced recognition, efficient cellular uptake, improved visualization of TK1 mRNA, and gene silencing with the hand-in-hand DNA tile assembly. Furthermore, specific drug delivery and enhanced cell killing were achieved using Dox as a chemotherapeutic model with target cells.
In situ stimuli-responsive molecular devices have gained much attention in biomedical areas due to their characteristics of increased image contrast and drug accumulation. Herein, we present a hand-in-hand in situ tile assembly for improved visualization of TK1 mRNA and killing of cancer cells. A pH-responsive and aptamer-functionalized tile motif (pH-Apt-TM) was first formed by four single-strand DNA, possessing pH-responsiveness and intracellular TK1 mRNA recognition capacity. When encountering target cells, the pH-Apt-TM could recognize target receptors on the cell surface through the aptamer domain. Meanwhile, the extracellular acidic pH gathered the pH-Apt-TM into a multifunctional hand-in-hand DNA tile assembly (HDTA) on the cells' surface. Compared to the pH-Apt-TM, studies revealed that the HDTA exhibited enhanced recognition, efficient cellular uptake, and improved visualization of TK1 mRNA, accompanied by gene silencing. Moreover, using Dox as a chemotherapeutic model, specific drug delivery and enhanced cell killing were achieved with target cells.
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