期刊
ANALYTICAL CHEMISTRY
卷 93, 期 31, 页码 10940-10946出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c01814
关键词
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资金
- Johns Hopkins University Whiting School of Engineering
- China National Science and Technology Infrastructure Program [2018YFC0840405]
African swine fever (ASF), with a nearly 100% mortality rate, has devastated pork industries around the world. In the absence of a vaccine, a portable device has been developed for rapid ASF detection, exhibiting high diagnostic accuracy in farm and slaughter facility settings.
With a nearly 100% mortality rate, African swine fever (ASF) has devastated the pork industry in many countries. Without a vaccine in sight, mitigation rests on rapid diagnosis and immediately depopulating infected or exposed animals. Unfortunately, current tests require centralized laboratories with well-trained personnel, take days to report the results, and thus do not meet the need for such rapid diagnosis. In response, we developed a portable, sample-to-answer device that allows for ASF detection at the point of need in <30 min. The device employs droplet magnetofluidics to automate DNA purification from blood, tissue, or swab samples and utilizes fast thermal cycling to perform real-time quantitative polymerase chain reaction (qPCR), all within an inexpensive disposable cartridge. We evaluated its diagnostic performance at six farms and slaughter facilities. The device exhibits high diagnostic accuracy with a positive percent agreement of 92.2% and a negative percent agreement of 93.6% compared with a lab-based reference qPCR test.
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