期刊
ANALYTICAL CHEMISTRY
卷 93, 期 33, 页码 11617-11625出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02388
关键词
-
资金
- National Natural Science Foundation of China [21775068, 21974066, 21475060, 21227009]
- Excellent Research Program of Nanjing University [ZYJH004]
The study utilized cascaded DNA circuits on a bead array platform for signal amplification of miRNA, achieving high sensitivity detection in single cells. This method allows accurate quantification and identification of single nucleotide differences, providing new possibilities for disease treatment strategies.
Single-cell microRNA (miRNA) analysis helps people understand the causes of diseases and formulate new disease treatment strategies. However, miRNA from a single cell is usually very rare and requires signal amplification for accurate quantification. Here, to amplify the signal, we constructed the cascaded DNA circuits consisting of catalytic hairpin assembly and hybrid chain reaction into the bead array platform, on which the uniformly distributed beads were adopted for miRNA quantification. After exponential signal amplification, a consistent linear correlation between the percentage of fluorescent beads and the copy number of miRNA was detected. The proposed bead array can achieve ultrahigh sensitivity as low as 60 copies of miR-155 and high specificity for distinguishing single nucleotide differences. This method has been successfully applied to the quantitative detection of miRNA in a single cancer cell. The high sensitivity, programmability, and simple workflow of the bead array chip will give a huge advantage in basic and clinical research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据