期刊
ANALYTICAL CHEMISTRY
卷 93, 期 32, 页码 11312-11320出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02670
关键词
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资金
- National Natural Science Foundation of China [21775016, 21874013, 22074013]
- Fundamental Research Funds for the Central Universities [N2005027, N2105018]
- Talent Project of Revitalizing Liaoning [XLYC1807165]
The study evaluated a high-performance solid-state ECL H2O2 sensor based on MIL-88B(Fe) nanocrystal-anchored Ti microwires, showing ultrahigh sensitivity, long-term stability, and excellent photocatalytic activity in the visible-light range at the single-cell level. The electrode demonstrated synergistic effects between the TiO2 luminophore and the peroxidase-like activity of MIL-88B(Fe), making it suitable for cytosensing applications.
As one of the significant intracellular signaling molecules, hydrogen peroxide (H2O2) regulates some vital biological processes. However, it remains a challenge to develop noninvasive electrodes that can be used for sensing trace H2O2 at the cellular level. Here, we evaluated a high-performance solid-state electrochemiluminescence (ECL) H2O2 sensor based on MIL-88B(Fe) nanocrystal-anchored Ti microwires. Semiconducting TiO2 nanotubes (TiNTs) vertically grown around a Ti wire via an anodization technique act as an intrinsic ECL luminophore. By integrating with MIL-88B(Fe), the synergistic effect of the TiO2 luminophore and the remarkable peroxidase-like activity of MIL-88B(Fe) enable the resulting H2O2 sensor an ultrahigh sensitivity featuring a minimum detection limit of 0.1 nM (S/N = 3), long term stability, high durativity, and wide-range linear response to a concentration of up to 10 mM. To demonstrate the concept of a MIL-88B(Fe)gTiO(2) microelectrode for single-cell sensing, the electrode was used to detect intracellular H2O2 in a single cell. Moreover, benefiting from the heterojunction of MIL-88B(Fe)/TiO2, the microelectrode was found to exhibit excellent photocatalytic activity in the visible-light range, that is, the sensor surface can be self-cleaning after a short visible-light treatment. These advanced sensor characteristics involving easy reusability reveal that the MIL-88B(Fe)@aTiO(2) microelectrode is a new platform for cytosensing. This study provides a new strategy to design semiconductor materials with arbitrary shape and size, allowing for profound applications in biomedical and clinical analysis.
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