期刊
ANALYTICAL BIOCHEMISTRY
卷 625, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2021.114222
关键词
Cry2Aa toxin; Anti-idiotypic antibody; Single-chain variable fragment (scFv)
资金
- National Natural Science Foundation of China [31630061, 31972292]
- Jiangsu Agricultural Independent Innovation Fund [CX(20)3053]
- Natural Science Foundation of Jiangsu Province [BK20181247]
- National Key Research and Development Program of China [2019YFE0103900]
- European Union's Horizon 2020 Research and Innovation Program [861917]
The study obtained seven anti-Cry2Aa toxin anti-idiotypic scFv antibodies from a phage-displayed mutant library, with mutant 2-12B showing the best binding ability. 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs and had seven common binding amino acid residues in polycalin. Insect bioassay results indicated that 2-12 had insecticidal efficacy against P. xylostella larvae, suggesting that the 2-12B antibody partially mimics the structure and function of Cry2Aa toxin.
The anti-idiotypic antibody is widely used in the field of immunology to simulate structural features or even induce the biological activity of antigens. In this study, we obtained seven anti-idiotypic single-chain variable fragments (scFv) antibodies of Cry2Aa toxin from a phage-displayed mutant library constructed using errorprone PCR technique. A mutant designated 2-12B showed the best binding ability amongst all anti-idiotypic scFv isolates to Plutella xylostella brush border membrane vesicles (BBMVs). 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs. Homology modeling and molecular docking demonstrated that 2-12B and Cry2Aa toxin have seven common binding amino acid residues in polycalin. Insect bioassay results suggested that 2-12 had insecticidal efficacy against P. xylostella larvae. These results indicated that the Cry2Aa anti-idiotypic scFv antibody 2-12B partially mimicked the structure and function of Cry2Aa toxin. The anti-idiotypic scFv antibody provides the basic material for the future study of surrogate molecules or new insecticidal materials.
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