期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 23, 页码 5733-5742出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03548-5
关键词
Palladium nanoparticles; Nanozyme; Enzyme-linked immunosorbent assay; Lateral flow immunoassay; Glycocholic acid
资金
- Guangzhou Science and Technology Foundation [2016201604030025, 201902010430100011]
- Guangdong Science and Technology Foundation [2017A050501034, 2018A030313926, 2019B020209009]
The study demonstrates the potential of PdNPs as a labeling matrix in enzyme-free single-step immunoassays, showing good catalytic performance and the ability to replace natural bio-enzymes as labeling tags for efficient and sensitive detection of GCA.
Palladium nanoparticles (PdNPs) are composed mainly of inert noble metals, and their outstanding properties have attracted wide attention. PdNPs are not only capable of mimicking the oxidase-like characteristics of natural bio-enzymes, but they also present a clear black band in the test zone. In this work, the synthesized PdNPs promoted a transformation of colorless tetramethylbenzidine (TMB) to a blue oxidation product of TMB, providing a K-m value of 0.09 mM for TMB, and revealing the good catalytic performance of the synthesized PdNPs. For both signal generation and amplification, PdNPs effectively replaced natural bio-enzymes as a new labeling tag. Thus, the PdNP-based enzyme-free single-step immunoassays were successfully developed for efficient and sensitive detection of glycocholic acid (GCA). Under optimal conditions, a noticeable linear relationship was identified by the enzyme-linked immunosorbent assay (ELISA) over a range of 8-2390 ng/mL, while the visual limit of detection (vLOD) in the constructed lateral flow immunoassay (LFA) was 10 ng/mL for GCA. The recovery rate in spiked urine samples obtained by the ELISA ranged from 84.2 to 117.9%, which was consistent with the results in LFA. The present work demonstrates the potential of PdNPs as labeling matrices in enzyme-free single-step immunoassays.
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