4.7 Article

A near-infrared fluorescent probe with large Stokes shift for imaging Cys in tumor mice

期刊

ANALYTICA CHIMICA ACTA
卷 1171, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.aca.2021.338655

关键词

Fluorescent probe; Cys; Near-infrared; Large Stokes shift; Tumor mice

资金

  1. National Natural Science Foundation of China [21775133]
  2. Scientific Research Fund of Hunan Provincial Education Department [19A479]
  3. Open Research Fund of School of Chemistry and Chemical Engineering, Henan Normal University [2020YB01]
  4. Key Project of Science and Technology of Henan Province [202102310215]
  5. Degree & Postgraduate Education Reform Project of Hunan Province [2019JGYB113]
  6. Hunan Provincial Innovation Foundation For Postgraduate [CX20190483, XDCX2020B115]

向作者/读者索取更多资源

A near-infrared fluorescent probe named IX has been designed and synthesized for detecting Cysteine (Cys) with high sensitivity and selectivity. The fluorescence enhancement mechanism involves the reaction of IX with Cys to release IX-OH, allowing for imaging of Cys in cells and tumor mice.
Cysteine (Cys), a kind of small molecule biological thiol, not only involves in the regulation of physiological processes, but also is considered a marker of tumor. However, it is challenging to develop suitable probe for detecting Cys in tumors. In this paper, a near-infrared (NIR) fluorescent probe named IX for Cys has been designed and synthesized. The probe shows a NIR emission peak at 770 nm with large Stokes shift (180 nm) upon adding Cys. It displays high sensitivity to Cys with 6-fold increase of fluorescence intensity. Meanwhile, IX has the high selectivity to Cys over other potential interference such as Hcy and GSH, which have similar structure with Cys. In addition, a possible mechanism of fluorescence enhancement is the reaction of IX with Cys to release IX-OH, which is verified by fluorescence spectra, MS and HPLC. Next, IX can selectively image Cys in HCT-116 cells thanks to the low cytotoxicity. Most important of all, the fluorescent probe IX has visualized Cys in HCT116-xenograft tumor mice due to the near-infrared properties with large Stokes shift. (c) 2021 Elsevier B.V. All rights reserved.

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