期刊
ANALYTICA CHIMICA ACTA
卷 1175, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2021.338671
关键词
Caenorhabditis elegans; Secondary ion mass spectrometry; Size-dependent uptake; Nanoparticle uptake; Single particle inductively coupled plasma; mass spectrometry; Gold nanoparticles
The study combined dynamic SIMS and spICP-MS techniques to determine the biodistribution and quantity of gold nanoparticles ingested by Caenorhabditis elegans. The complementary data from SIMS image depth profiling and spICP-MS provided a complete view of the uptake, translocation, and size distribution of ingested NPs within Caenorhabditis elegans.
Analytical techniques capable of determining the spatial distribution and quantity (mass and/or particle number) of engineered nanomaterials in organisms are essential for characterizing nano-bio interactions and for nanomaterial risk assessments. Here, we combine the use of dynamic secondary ion mass spectrometry (dynamic SIMS) and single particle inductively coupled mass spectrometry (spICP-MS) techniques to determine the biodistribution and quantity of gold nanoparticles (AuNPs) ingested by Caenorhabditis elegans. We report the application of SIMS in image depth profiling mode for visualizing, identifying, and characterizing the biodistribution of AuNPs ingested by nematodes in both the lateral and z (depth) dimensions. In parallel, conventional-and sp-ICP-MS quantified the mean number of AuNPs within the nematode, ranging from 2 to 36 NPs depending on the size of AuNP. The complementary data from both SIMS image depth profiling and spICP-MS provides a complete view of the uptake, translocation, and size distribution of ingested NPs within Caenorhabditis elegans. Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据