Reproducible, shelf-stable, and bioaffinity SERS nanotags inspired by multivariate polyphenolic chemistry for bacterial identification

Efficient identification of pathogenic bacteria is greatly concerned with microbial food safety and foodborne diseases diagnosis. Surface-enhanced Raman scattering (SERS) tags are among the cutting edge tools for bioanalysis, but facing troubles in either SERS sensitivity, durability, interfering signals, or universal recognition agents for target bacteria. This work proposed a multivariate scheme enabled by polyphenolic chemistry for the green synthesis, facile stabilization (functionalization), protective encapsulation, and bio-affinitive design of metal-phenolic networks (MPNs)-encapsulated silver SERS nanotags (AgNPs@4-mercaptobenzonitrile@MPNs). With remarkable SERS properties, shelf stability, and bacterial affinity, AgNPs@4-mercaptobenzonitrile@MPNs tags enabled rapid, reproducible, and interference-free SERS detection of two representative foodborne pathogens (i.e., E. coli O157: H7 and S. aureus) in the assistance of an aptamer-labelled magnetic probe, reaching good sensitivity and selectivity. Moreover, this SERS biosensor worked well in real food samples, manifesting the potential of polyphenolic chemistry in the customization of bio-affinitive SERS nanotags for food safety detection. (c) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Automated summary

A multivariate scheme enabled by polyphenolic chemistry was proposed for the green synthesis of metal-phenolic networks (MPNs)-encapsulated silver SERS nanotags, which showed remarkable SERS properties and bacterial affinity. This approach enabled rapid, reproducible, and interference-free SERS detection of target bacteria in food samples, demonstrating the potential of polyphenolic chemistry in food safety detection.