☆ 4.7 Review

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

ANALYTICA CHIMICA ACTA (2021)

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Article Biophysics

A CRISPR-derived biosensor for the sensitive detection of transcription factors based on the target-induced inhibition of Cas12a activation

Bingzhi Li et al.

Summary: Transcription factors (TFs) are crucial proteins for cell fate determination and have potential applications in disease diagnostics and treatment. A highly sensitive biosensor based on the CRISPR/Cas12a system was developed for TF detection, utilizing competitive binding between Cas12a-crRNA and TFs to a specific dsDNA activator. This study demonstrates a novel approach in applying CRISPR technology for TF sensing and highlights the potential of using blocking mechanisms as a new sensing strategy for CRISPR-based biosensors.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Chemistry, Multidisciplinary

Holographic Optical Tweezers and Boosting Upconversion Luminescent Resonance Energy Transfer Combined Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a Biosensors

Cheng-Yu Li et al.

Summary: The CRISPR/Cas12a system offers an alternative opportunity for designing fluorescence biosensors with outstanding precision in target recognition. By utilizing collateral cleavage and representative transduction pathways, the system can extend sensing coverage and improve assay sensitivity. The developed biosensors not only exhibit ultrasensitive behavior towards non-nucleic acid analytes but also show practical application capacity in real samples.

ACS NANO (2021)

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Article Chemistry, Multidisciplinary

An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics

Tian Tian et al.

Summary: The confinement effect-inspired Cas13a assay for single-molecule RNA diagnostics eliminates the need for nucleic acid amplification and reverse transcription, achieving a >10,000-fold enhancement in sensitivity and enabling absolute digital single-molecule RNA quantitation. Its broad applicability and accurate detection of various RNA molecules at the single-molecule level make it a potential dominant rival to RT-qPCR.

ACS NANO (2021)

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Article Chemistry, Analytical

High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA

Ting Zhou et al.

Summary: Exploring the trans-cleavage ability of Cas13a and developing a specific and sensitive visual detection system for miRNA, called vCas, with a detection limit of 1 fM and single-base specificity, suggests a promising application in clinical molecular diagnosis.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction

Mahera J. Kachwala et al.

Summary: Hybridization chain reaction (HCR) is a DNA-based target-induced cascade reaction that is often used for sensing applications due to its unique enzyme-free amplification feature. HCR is highly modular and can be advanced and repurposed when coupled with latest discoveries for improved sensitivity and programmability. Incorporating CRISPR-Cas12a into HCR can enhance sensitivity and enable rapid reprogramming of detection assays, making it more versatile for detecting different target sequences.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Simultaneous Dual-Gene Diagnosis of SARS-CoV-2 Based on CRISPR/Cas9-Mediated Lateral Flow Assay

Erhu Xiong et al.

Summary: The newly developed method can simultaneously detect two SARS-CoV-2 genes in a single test, showing high sensitivity and accuracy, providing a more accurate and convenient pathway for COVID-19 diagnosis in low-resource regions.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Biophysics

A CRISPR-driven colorimetric code platform for highly accurate telomerase activity assay

Meng Cheng et al.

Summary: The Telomeric Repeat Amplification Protocol (TRAP) is commonly used for assessing telomerase activity, but often faces challenges such as false-negative results due to inhibiting factors in cell lysates or tissue samples. A new colorimetric code system based on CRISPR-Cas12a and AuNPs probe has been developed to rapidly analyze telomeric repeat DNA in TRAP products, enabling easy detection of telomerase activity and false-negatives. This platform has shown promise in detecting telomerase activity in clinical liver cancer samples with high sensitivity and specificity. Furthermore, a Cas9-mediated lateral flow assay has been demonstrated as a convenient and accurate method for telomerase activity assay on a single test strip.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

Terminal deoxynucleotidyl transferase combined CRISPR-Cas12a amplification strategy for ultrasensitive detection of uracil-DNA glycosylase with zero background

Yi-Chen Du et al.

Summary: A highly sensitive biosensing strategy utilizing TdT and CRISPR-Cas12a for signal amplification was reported, showing great potential in the design of various biosensors and achieving ultrasensitive detection of UDG and T4 PNK. The biosensors developed based on this strategy display high sensitivity, signal amplification efficiency, and low background noise, making them promising for clinical diagnosis and biomedical research.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.

CELL (2021)

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Article Chemistry, Applied

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Hua Liu et al.

Summary: This study developed a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety, which can rapidly, simply, and highly sensitively detect foodborne pathogenic bacteria, genetically modified crops, and meat adulteration without requiring technical expertise or equipment.

FOOD CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

Jin-Ha Choi et al.

Summary: A non-nucleic acid amplification fluorescent biosensor using gold nanoparticles for metal-enhanced fluorescence detection of cfDNA has been developed in this study, allowing rapid and highly sensitive detection of BRCA-1. This sensor is highly selective and can be widely applied for the measurement of other nucleic acid biomarkers.

NANO LETTERS (2021)

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Article Chemistry, Multidisciplinary

Digital CRISPR/Cas-Assisted Assay for Rapid and Sensitive Detection of SARS-CoV-2

Joon Soo Park et al.

Summary: The digital-enhanced CRISPR/Cas-assisted one-pot virus detection (deCOViD) is a novel assay that allows qualitative and quantitative detection of SARS-CoV-2 in a very short time with high sensitivity and a broad dynamic range. It outperforms current benchtop-based counterparts and represents one of the fastest and most sensitive CRISPR/Cas-assisted SARS-CoV-2 detection methods to date.

ADVANCED SCIENCE (2021)

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Article Chemistry, Analytical

An ultrasensitive and point-of-care sensor for the telomerase activity detection

Xiaolong Chen et al.

Summary: Telomerase has great potential in cancer diagnosis and treatment, and a sensor has been developed for ultrasensitive detection of telomerase activity in MCF-7 cells, with potential for classification of breast cancer subtypes.

ANALYTICA CHIMICA ACTA (2021)

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Article Chemistry, Analytical

Detection of SARS-CoV-2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription Amplification

Yuxi Wang et al.

Summary: The detection method using light-up CRISPR-Cas13 technology can sensitively and accurately detect SARS-CoV-2 and its mutated variants, with high specificity and sensitivity, allowing for strict discrimination of key mutations.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Reverse Transcription Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection

Wei S. Zhang et al.

Summary: The proposed method combines RT-RPA with CRISPR-Cas12a colorimetric assay for SARS-CoV-2 detection, utilizing DNA-modified gold nanoparticles to achieve high sensitivity and specificity. This novel approach can effectively avoid false positive results from other beta coronavirus members, improving the practical testing availability of SARS-CoV-2.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria

Ting Zhang et al.

Summary: This study developed a new detection system that can accurately distinguish viable pathogenic bacteria without the need for reverse transcription, nucleic acid amplification, or labeled substrates. By utilizing CRISPR-Cas13a and a light-up RNA aptamer, it enables rapid detection and precise quantification of viable pathogens.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

CRISPR/Cas9 cleavage triggered ESDR for circulating tumor DNA detection based on a 3D graphene/AuPtPd nanoflower biosensor

Mei Chen et al.

Summary: A novel 3D GR/AuPtPd nanoflower sensing platform based on CRISPR/Cas9 cleavage-triggered entropy-driven strand displacement reaction (ESDR) was developed for effective detection of ctDNA. This method allows for low levels of ctDNA detection without requiring complicated operation procedures and stringent reaction conditions.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biotechnology & Applied Microbiology

A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection

Wei Lin et al.

Summary: The study introduced a CRISPR/Cas9 eraser strategy to eliminate the risk of contamination amplification in PCR. By engineering primers and pre-incubating Cas9/sgRNA, selective cleavage of contaminated products was achieved to improve the accuracy of virus detection.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

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Article Chemistry, Analytical

Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly

Ying Cui et al.

Summary: In this study, an ultrasensitive electrochemical biosensing platform for miRNA-21 assay was developed by combining the CRISPR-Cas13a system and catalytic hairpin assembly (CHA). The platform demonstrated high sensitivity with a low detection limit of 2.6 fM, showing great potential for early clinical diagnostic applications.

TALANTA (2021)

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Review Chemistry, Analytical

Versatile detection with CRISPR/Cas system from applications to challenges

Hui Wu et al.

Summary: The CRISPR/Cas system is playing an important role in biosensing applications, combining with multiple detection techniques for various targets detection. However, there are still challenges that need to be addressed in the future, such as off-target effect, target sequence limitation.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics

Richard Bruch et al.

Summary: MicroRNAs have become important biomarkers for various diseases in clinical and point-of-care diagnostics, but require consideration of other factors for accurate diagnosis. This study successfully designed and implemented multiplexed versions of an electrochemical microfluidic biosensor for simultaneous quantification of multiple miRNAs.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biochemical Research Methods

Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections

JingJing Wei

Summary: In this study, a novel method using CRISPR-Cas12a for sensitive and accurate detection of Staphylococcus aureus was developed, with high sensitivity and accuracy demonstrated. The proposed method has a potential for diverse sensing platforms for detecting different trace bacteria.

JOURNAL OF MICROBIOLOGICAL METHODS (2021)

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Article Biochemistry & Molecular Biology

Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR-Cas12f nuclease

Renjian Xiao et al.

Summary: Cas12f, also known as Cas14, is a small type V-F CRISPR-Cas nuclease with unique substrate recognition and activation mechanisms. Cryo-EM structures revealed an asymmetric Cas12f dimer bound to sgRNA for dsDNA cleavage, highlighting the importance of coordinated conformational changes induced by substrate recognition. Additionally, truncated sgRNA was found to be active for target DNA cleavage, indicating potential genome editing applications for the miniature Cas12f.

NUCLEIC ACIDS RESEARCH (2021)

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Article Chemistry, Analytical

An ultrasensitive and point-of-care strategy for enzymes activity detection based on enzyme extends activators to unlock the ssDNase activity of CRISPR/Cas12a (EdU-CRISPR/Cas12a)

Xiaolong Chen et al.

Summary: Enzymes are essential components of organisms, ensuring normal life activities. Abnormal enzyme activity can lead to dysfunction and disease. Establishing simple, sensitive, and timely detection of enzyme activity is crucial for biomedical testing and disease diagnosis and treatment. We developed a strategy for detecting T4 PNK, telomerase, or APE1 activity using enzyme extends activators to unlock CRISPR/Cas12a's ssDNase activity (EdU-CRISPR/Cas12a), achieving low detection limits through fluorescence and lateral flow assay methods. Our approach has the potential to greatly improve enzyme activity detection.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Review Chemistry, Analytical

CRISPR-Cas systems: From gene scissors to programmable biosensors

Yuxin Zhang et al.

Summary: The increasing demand for developing biosensors in various fields poses challenges on sensor performance requirements such as sensitivity, selectivity, and operating costs. CRISPR-Cas systems, known as molecular scissors, are highly accurate in cleaving specific DNA and RNA sequences, and recent studies have shown the establishment of versatile biosensing platforms based on CRISPR-Cas. The review introduces the general characteristics and mechanisms of class II CRISPR-Cas systems, highlighting their biosensing strategies for detecting a wide range of nucleic acid and non-nucleic acid analytes.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Article Multidisciplinary Sciences

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

Kean Hean Ooi et al.

Summary: Extensive testing is crucial to control the spread of SARS-CoV-2 in the ongoing COVID-19 pandemic. The authors have developed a CRISPR-based diagnostic assay that can detect both wildtype and known variants of the virus efficiently and quickly.

NATURE COMMUNICATIONS (2021)

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Review Chemistry, Multidisciplinary

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

Wei Feng et al.

Summary: The advancement of CRISPR-Cas technology has revolutionized genome editing and transcriptional modulation, as well as molecular detection of diverse targets. Integrating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, improved specificity and sensitivity, and development of point-of-care diagnostic techniques.

CHEMICAL SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip

Yong Chen et al.

Summary: This study presents a novel SNP detection method using the CRISPR system to improve universality, sensitivity, and specificity, successfully applied in clinical blood samples. This genotyping method can rapidly detect multiple genotypes in a short period of time.

CHEMICAL SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay

Yongya Li et al.

Summary: The isothermal proximity CRISPR Cas12a assay utilizes the target-induced single-stranded DNase activity of Cas12a for quantitative gene expression profiling and sensitive protein detection. It achieves high sensitivity and specificity through proximity binding instead of recognition by CRISPR RNA, allowing for flexible assay design. This method demonstrated practical applicability by successfully profiling interleukin-6 gene expression during allergen-mediated mast cell activation.

CHEMICAL SCIENCE (2021)

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Article Multidisciplinary Sciences

A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics

Kai Shi et al.

Summary: CRISPR-Cas-only amplification network (CONAN) utilizes a Cas12a-driven reaction network to construct a positive feedback circuit, enabling isothermally amplified detection of genomic DNA with attomolar sensitivity, showcasing its potential as a powerful tool for disease diagnostics.

SCIENCE ADVANCES (2021)

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Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive

Hui Wu et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Chemistry, Multidisciplinary

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

Xusheng Wang et al.

ACS NANO (2020)

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Rapid and sensitive exosome detection with CRISPR/Cas12a

Xianxian Zhao et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

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CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection

Qian Chen et al.

ANALYTICAL CHEMISTRY (2020)

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Rolling Circular Amplification (RCA)-Assisted CRISPR/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs

Ruixuan Wang et al.

ANALYTICAL CHEMISTRY (2020)

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Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

Chaoqun Yuan et al.

ANALYTICAL CHEMISTRY (2020)

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Review Chemistry, Multidisciplinary

CRISPR-Cas System for RNA Detection and Imaging

Chen Siyu et al.

CHEMICAL RESEARCH IN CHINESE UNIVERSITIES (2020)

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Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

Ying Xiong et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

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Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time

Brandon J. Davis et al.

MOLECULAR CELL (2020)

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Kira S. Makarova et al.

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Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

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Integration of logic gates to CRISPR/Cas12a system for rapid and sensitive detection of pathogenic bacterial genes

Lei Peng et al.

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Dynamic Aqueous Multiphase Reaction System for One-Pot CRISPR-Cas12a-Based Ultrasensitive and Quantitative Molecular Diagnosis

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ANALYTICAL CHEMISTRY (2020)

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High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

Qian He et al.

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Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics

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Ting Zhou et al.

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Michael M. Kaminski et al.

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PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage

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Jin Zhou et al.

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CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs

Zihni Onur Uygun et al.

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Yifan Dai et al.

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Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement

Yaya Hao et al.

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An ultrasensitive and specific point-of-care CRISPR/Cas12 based lateral flow biosensor for the rapid detection of nucleic acids

Omar Mukama et al.

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End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor

Hui Wu et al.

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Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplification

Liqi Xu et al.

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Jeanne E. van Dongen et al.

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Terminal deoxynucleotidyl transferase induced activators to unlock the trans-cleavage of CRISPR/Cpf 1 (TdT-IU- CRISPR/Cpf 1): An ultrasensitive biosensor for Dam MTase activity detection

Xiaolong Chen et al.

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Meng Wang et al.

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Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

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MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection

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Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9

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Yue Li et al.

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David F. Savage

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Weidan Chang et al.

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Peiwu Qin et al.

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CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic

Guillermo Aquino-Jarquin

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CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

Yi Li et al.

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Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp

Thawatchai Chaijarasphong et al.

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Uracil-Mediated New Photospacer-Adjacent Motif of Cas12a To Realize Visualized DNA Detection at the Single-Copy Level Free from Contamination

Cheng Qian et al.

ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay

Yongya Li et al.

ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection

Bei Wang et al.

ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip

Ning Shao et al.

ANALYTICAL CHEMISTRY (2019)

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Article Biochemical Research Methods

SHERLOCK: nucleic acid detection with CRISPR nucleases

Max J. Kellner et al.

NATURE PROTOCOLS (2019)

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Article Multidisciplinary Sciences

CRISPR-mediated live imaging of genome editing and transcription

Haifeng Wang et al.

SCIENCE (2019)

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Article Biochemical Research Methods

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Linxian Li et al.

ACS SYNTHETIC BIOLOGY (2019)

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Article Chemistry, Multidisciplinary

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

Yifan Dai et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

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Article Multidisciplinary Sciences

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

Mindong Liang et al.

NATURE COMMUNICATIONS (2019)

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Review Biotechnology & Applied Microbiology

CRISPR/Cas Systems towards Next-Generation Biosensing

Yi Li et al.

TRENDS IN BIOTECHNOLOGY (2019)

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Article Microbiology

Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever

Jing Bai et al.

FRONTIERS IN MICROBIOLOGY (2019)

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Article Biochemical Research Methods

Detection of target DNA with a novel Cas9/sgRNAs-associated reverse PCR (CARP) technique

Beibei Zhang et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2018)

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Review Chemistry, Multidisciplinary

Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies

Spencer C. Knight et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

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Letter Cell Biology

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

Shi-Yuan Li et al.

CELL RESEARCH (2018)

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Review Chemistry, Multidisciplinary

Optical nano-biosensing interface via nucleic acid amplification strategy: construction and application

Hong Zhou et al.

CHEMICAL SOCIETY REVIEWS (2018)

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Article Biotechnology & Applied Microbiology

Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes

Zhen Liang et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

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Article Multidisciplinary Sciences

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Digvijay Singh et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

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Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

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Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

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Article Chemistry, Analytical

CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases

Bonhan Koo et al.

SENSORS AND ACTUATORS B-CHEMICAL (2018)

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Review Multidisciplinary Sciences

The CRISPR tool kit for genome editing and beyond

Mazhar Adli

NATURE COMMUNICATIONS (2018)

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Article Multidisciplinary Sciences

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Yongmoon Jeon et al.

NATURE COMMUNICATIONS (2018)

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Article Biochemical Research Methods

Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9

Xin-Yuan Qiu et al.

ACS SYNTHETIC BIOLOGY (2018)

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Letter Cell Biology

CRISPR-Cas12a-assisted nucleic acid detection

Shi-Yuan Li et al.

CELL DISCOVERY (2018)

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Article Biochemical Research Methods

Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique

Beibei Zhang et al.

ANALYTICAL BIOCHEMISTRY (2018)

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Article Chemistry, Multidisciplinary

Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay

Kaixiang Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

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Article Multidisciplinary Sciences

CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method

Qiao Wang et al.

SCIENTIFIC REPORTS (2018)

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Article Multidisciplinary Sciences

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Lucas B. Harrington et al.

SCIENCE (2018)

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Article Multidisciplinary Sciences

A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

Wenhua Zhou et al.

NATURE COMMUNICATIONS (2018)

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Article Biochemical Research Methods

Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

Yihao Zhang et al.

ACS SYNTHETIC BIOLOGY (2017)

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Article Multidisciplinary Sciences

RNA targeting with CRISPR-Cas13

Omar O. Abudayyeh et al.

NATURE (2017)

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Review Microbiology

Diversity and evolution of class 2 CRISPR-Cas systems

Sergey Shmakov et al.

NATURE REVIEWS MICROBIOLOGY (2017)

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Article Biochemistry & Molecular Biology

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme

Gavin J. Knott et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2017)

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Article Biochemistry & Molecular Biology

CUT-PCR: CRISPR-mediated, ultrasensitive detection of target DNA using PCR

S. H. Lee et al.

ONCOGENE (2017)

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Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

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Review Chemistry, Multidisciplinary

Sensing telomerase: From in vitro detection to in vivo imaging

Li-juan Wang et al.

CHEMICAL SCIENCE (2017)

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Article Biochemistry & Molecular Biology

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme

Gavin J. Knott et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2017)

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Article Chemistry, Analytical

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

Mengqi Huang et al.

ANALYTICAL CHEMISTRY (2017)

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Article Biophysics

A facile, rapid and sensitive detection of MRSA using a CRISPR-mediated DNA FISH method, antibody-like dCas9/sgRNA complex

Kyeonghye Guk et al.

BIOSENSORS & BIOELECTRONICS (2017)

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Article Biochemistry & Molecular Biology

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a

Liang Liu et al.

CELL (2017)

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Review Microbiology

Diversity, classification and evolution of CRISPR-Cas systems

Eugene V. Koonin et al.

CURRENT OPINION IN MICROBIOLOGY (2017)

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Article Chemistry, Multidisciplinary

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

Piyush K. Jain et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016)

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Article Biochemistry & Molecular Biology

PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease

Hui Yang et al.

CELL (2016)

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Review Microbiology

Sensing new chemicals with bacterial transcription factors

Vincent Libis et al.

CURRENT OPINION IN MICROBIOLOGY (2016)

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Article Multidisciplinary Sciences

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor et al.

NATURE (2016)

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Article Multidisciplinary Sciences

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

Alexandra East-Seletsky et al.

NATURE (2016)

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Article Multidisciplinary Sciences

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Omar O. Abudayyeh et al.

SCIENCE (2016)

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Article Chemistry, Multidisciplinary

Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification

Kaixiang Zhang et al.

CHEMICAL SCIENCE (2016)

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Article Biochemistry & Molecular Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche et al.

CELL (2015)

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Review Chemistry, Multidisciplinary

Isothermal Amplification of Nucleic Acids

Yongxi Zhao et al.

CHEMICAL REVIEWS (2015)

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Article Chemistry, Multidisciplinary

Optical Control of CRISPR/Cas9 Gene Editing

James Hemphill et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

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Review Microbiology

An updated evolutionary classification of CRISPR-Cas systems

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2015)

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Article Chemistry, Multidisciplinary

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

Kuangwen Hsieh et al.

CHEMICAL COMMUNICATIONS (2014)

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Review Chemistry, Multidisciplinary

Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine

M. Monsur Ali et al.

CHEMICAL SOCIETY REVIEWS (2014)

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Article Biochemical Research Methods

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

Bin Shen et al.

NATURE METHODS (2014)

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Article Biochemical Research Methods

Real-time, label-free isothermal solid-phase amplification/detection (ISAD) device for rapid detection of genetic alteration in cancers

Yong Shin et al.

LAB ON A CHIP (2013)

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Article Multidisciplinary Sciences

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali et al.

SCIENCE (2013)

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Article Multidisciplinary Sciences

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong et al.

SCIENCE (2013)

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Review Biotechnology & Applied Microbiology

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

Thomas Gaj et al.

TRENDS IN BIOTECHNOLOGY (2013)

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Article Multidisciplinary Sciences

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Giedrius Gasiunas et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

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Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

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Review Chemistry, Multidisciplinary

Dynamic DNA nanotechnology using strand-displacement reactions

David Yu Zhang et al.

NATURE CHEMISTRY (2011)

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Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

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Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

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Article Biochemistry & Molecular Biology

T7 RNA polymerase mediates fast promoter-independent extension of unstable nucleic acid complexes

HS Zaher et al.

BIOCHEMISTRY (2004)

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Article Multidisciplinary Sciences

Isothermal reactions for the amplification of oligonucleotides

J Van Ness et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

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Article Biochemistry & Molecular Biology

Identification of genes that are associated with DNA repeats in prokaryotes

R Jansen et al.

MOLECULAR MICROBIOLOGY (2002)

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Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)

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