期刊
ADVANCED FUNCTIONAL MATERIALS
卷 31, 期 44, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202101003
关键词
agriculture; nanotechnology; RNAi; siRNA; viral diseases
类别
资金
- European Union [908049, 680242]
- Technion Integrated Cancer Center (TICC)
- Russell Berrie Nanotechnology Institute
- Lorry I. Lokey Interdisciplinary Center for Life Sciences Engineering
- Israel Ministry of Economy [52752, 69230]
- Israel Ministry of Science Technology and Space -Office of the Chief Scientist [3-11878]
- Israel Innovation Authority for Nofar Grant [67967]
- Israel Science Foundation [1778/13, 1421/17]
- Israel Ministry of Science Technology [3-16963]
- Ministry of Agriculture & Rural Development -Office of the Chief Scientist [323/19]
- Israel Ministry of Science and Technology [3-17418]
- Israel Cancer Association [2015-0116]
- Leventhal 2020 COVID19 Research Fund [11947]
- German-Israeli Foundation for Scientific Research and Development [I-23281139.10/2012]
- Phospholipid Research Center Grant [ASC-2018-062/1-1]
- Louis family Cancer Research Fund, a Mallat Family Foundation Grant
- Unger Family Fund
- Carrie Rosenblatt Cancer Research Fund
- Alon Fellowship
- Taub Fellowship
- European Research Council (ERC) [680242] Funding Source: European Research Council (ERC)
The study successfully utilized RNA interference (RNAi) technology to treat GLRaV-3 infection in Cabernet Sauvignon grapevines, effectively reducing viral titer and promoting recovery of the vines and grapes.
Grapevine leafroll disease (GLD) is a globally spreading viral infection that causes major economic losses by reducing crop yield, plant longevity, and berry quality, with no effective treatment. Grapevine leafroll associated virus-3 (GLRaV-3) is the most severe, prevalent GLD strain affecting wine production. Here, the ability of RNA interference (RNAi), a non-GMO gene-silencing pathway, to treat GLRaV-3 in infected Cabernet Sauvignon grapevines is evaluated. Lipid-modified polyethylenimine (lmPEI) is synthesized as the carrier for long double-stranded RNA (dsRNA, 250-bp-long) that targets RNA polymerase and coat protein is a gene target that are conserved in the GLRaV-3 genome. Self-assembled dsRNA-lmPEI particles, 220 nm in diameter, display inner ordered domains spaced 7.3 +/- 2 nm from one another, correlating to lmPEI wrapping spirally around the dsRNA. The particles effectively protect RNA from degradation by ribonucleases and show to increase uptake rate into plant cells as a result of the lipid component comprising the RNA carrier. In three field experiments, a single dose of foliar sprayed treatment of the RNA-particles knocks down GLRaV-3 titer, and multiple doses of the treatment keep the viral titer at baseline and trigger recovery of the vine and berries. This study demonstrates RNAi as a promising platform for treating viral diseases in agriculture.
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